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14 Periodical examination of hepatic or renal function and blood picture is desirable since Kedacillin may increase SGOT.

磺芐青霉素钠可使血清谷-草转氨霉，血清谷-丙转氨霉和血液尿素氮增高，使红细胞和白细胞减少。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Turn board that day, net dragon chairman holds heart of executive trustee Liu concurrently to be built to the reporter to express, net dragon hopes to borrow this to turn board attract compose of large scale computer and retail investor, increase trade turnover.

转板当日，网龙主席兼执行董事刘德建向记者表示，网龙希望借此次转板吸引大型机构及零售投资者，并增加交易流通量。

Objective To study the effect of Borna disease virus infection on the transcription of monoaminergic receptor genes in the brain tissues of neonatally inoculated rats.

目的分析博尔纳病病毒感染对新生大鼠脑内单胺类受体基因转录的影响。方法选择病毒滴度为2·0×106FFU/ml的BDV病毒液对新生大鼠进行颅内接种，接种量为10μl/只新生大鼠。30d后用RT-PCR和间接免疫荧光方法确定BDV感染情况；并采用半定量RT-PCR方法检测BDV感染大鼠脑组织中多巴胺2(D2)受体和5-羟色胺2α(5-HT2α)受体基因的mRNA转录情况。

IPhone's network users to switch defect must be die-hard Apple fans, only the iron in order to abandon the existing number of investment and the original operator's brand loyalty, and only iron in order to ignore the replacement cost and is bound to increase new business expenses, transfer network users will be the main body of iPhone.

投奔iPhone的转网用户一定是铁杆的苹果粉丝，只有铁杆才能放弃既有的号码投资和对原运营商的品牌忠诚，也只有铁杆才能无视换机成本和必定增加的新业务支出，转网用户将是iPhone的主体。

In the standard measurement, such as bar code through a splitter, will be divided into two beams of light, a bunch of switch hitting the sensor photodiode array, and the other a bunch of change reach all parts of the observation mirror.

在水准测量中，条码的像通过一个分光器，将光线分成两束，一束转射到传感光电二极管阵列上，另一束转射到观测镜上。

To elucidate the biological structure basis of transgenic rice plants expressing maize PEPC gene with high photosynthesis efficiency, PEPC transgenic rice and its wild type with at 3 leaves seedling stage were treated under 1000 μmol/(m^2s) light intensity for 100 min, respectively, and those under 200 μmol/(m^2s) light as the control. The structure of mesophyll cells, bundle sheath and thylakoids of the second blade were observed with a transmission electron microscope.

为了阐明转玉米PEPC基因水稻高光效的结构基础，以转玉米PEPC基因水稻和未转基因原种为试验材料，在水稻的三叶期，施以100min，1000μmol/的高光强处理，以200μmol下的水稻为对照，运用透射电镜观察叶肉细胞、维管束鞘以及叶绿体内类囊体片层结构。

Torque control mechanism: Uses the worm bearing adjuster axial to flee moves, on the worm shaft is loaded with the small dish reed, flees the worm bearing adjuster axial moves through the crank drive to the torque axis, again touches the switch through the cam the principle.

4转矩控制机构：采用蜗杆轴向窜动，蜗杆轴上装有碟簧、把蜗杆的轴向窜动通过曲柄传动到转矩轴，再通过凸轮触动开关的原理。

At the same time ,the cap of filter paper was used for improving transformation ratio. Leaf discs were transferred onto the media MS+ NAA(1mg/L)+6-BA(3mg/L)+Km(30mg/L)for regeneration resistant selction. Shoots from selection medium were cultured on medium MS +NAA(1mg/L)+6-BA(3mg/L)+Km(50mg/L) for selection again,and then on the media 1/2MS+NAA(0.2mg/L)+IBA(0.2mg/L)+Km(25mg/L)for rooting selectin.

共培养后把叶盘转入MS+ NAA(1mg/L)+6-BA(3mg/L)+Km(30mg/L)培养基上进行分化筛选培养。30天后可分化出不定芽，把不定芽转到MS +NAA(1mg/L)+6-BA(3mg/L)+Km(50mg/L)培养基上进行抗性芽的筛选，转到1/2MS+NAA(0.2mg/L)+IBA(0.2mg/L)+Km(25mg/L)培养基上进行生根筛选。

In the car movement control, this design uses a branch electrical machinery to control the car the advance and the backlash, step-by-steps the electrical machinery to control the car counterclockwise and the right-turn.

在小车的运动控制中，本设计采用一个支流电机控制小车的前进和后退，一个步进电机控制小车的左转和右转。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了