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We observed that FuGene6 regent had the highest transfection efficiency and lowest toxicity to auditory neurons in the three groups. The transfection efficiency was about 3%-10%, variable from experiment to experiment. However, when we used recombinant adenovirus vector to transfer LacZ gene into auditory neurons,β-gal expression was detected in more than 70% of both neurons and gilas at 100M0I viral concentration..

通过对不同染方法的选择和染条件的优化,其中FuGene 6介导的质粒染,其染效率最高,可达3%-10%左右,但不同批次结果重复性较差,易受不同批次细胞来源动物的个体差异及培养条件等因素的影响。

It is discussed that the phase inversion point of wet crude oil,the temperature and water ratio influence to the viscosity based on the lab experiment.

特超稠油相点的含水率约为50%,在相点以前,黏度随含水率的升高而增大;在相点处,黏度达到最大值;在相点后,黏度随含水率的升高而减小。

Consequences of the rotation of DNA are illustrated in Figure 9.17 in the twin domain model for transcription. As RNA polymerase pushes forward along the double helix, it generates positive supercoils ahead and leaves negative supercoils behind. For each

超螺旋与录还有更多的关系,RNA聚合酶录DNA时,解链和复链同时也发生,如同Fig113中所示,这需要整个录复合体绕DNA旋或者DNA自己绕它的双链轴旋,DNA旋的后果如Fig11.19所示,图中是一个录的双结构域模型,当RNA聚合酶沿DNA前移时,在酶前产生正双螺旋,酶后为负的双螺旋;每一个双螺旋角被RNA跨越后都会在前面产生+1角,后面产生-1角。

The sub-micron patterns can even be transferred onto the surface of polymer substracts at room temperature﹗ For replicating microstructure with deeper patterns, higher temperature and absorption pressure are needed.

对於印次微米深度之微结构,在常温下(23℃),即可达完整印;印较深之微结构,可藉著提升制程温度与渗入压力,成功印微结构。

The presence and absence of γ-glutamyl transpeptidases in sprouting and dormant garlic bulbs were determined.The relation between the activity of γ-glutamyl transpeptidases in the garlic and greening of garlic purees was investigated.

通过测定休眠与打破休眠的大蒜中γ-谷氨酰肽酶的活性存在与否,大蒜中γ-谷氨酰肽酶活性的变化与蒜泥绿变的关系,以及在不能绿变的大蒜中添加γ-谷氨酰肽酶而使蒜泥变绿,确定了γ-谷氨酰肽酶对蒜泥绿变的作用。

Overview Main Products containing the following: pad printer series 1, paragraph 58; monochrome to eight-color 2 screen printing machine series 38; cap / bottle / barrel surface silk screen printing machine automatic bottle machine hoses UV printing press 3 tipping machine, heat transfer machine Series 28 paragraph: fully automatic bottle cap shaped bottle gilt, gilt barrels / heat transfer machine 4 surrounding equipment series: 68 paragraph Air compressor Flame processor machine UV light solid metal etching machine net machine plate screen washing machine oven / Board furnace cabinet plastic injector sandblasting machine polishing mechanism grinder plate printing head scratching equipment grinding machine plastic ink viscosityframe transfer device beater trademark printing machine ink dye transfer gas water coated paper transfer equipment to hot / water transfer paper printing machine conveyor equipment / Heat Transfer Machine 5 Agents for sale:: imported ink - Germany Marabu MA to Po; French Dolby Dubuit; British Apollo Apollo; British Coates Gaoshi, etc.; France T3, Switzerland Sefar tournament yarn hair net;France watts, such as plastic scraper.

主要产品含概如下: 1移印机系列 58款;单色至八色 2丝印机系列 38款;瓶盖/瓶子/桶面丝印机全自动印瓶机UV软管印刷机· 3烫金机、热印机系列 28款:·全自动异形瓶盖烫金·酒瓶盖、桶烫金/热印机· 4周边设备系列: 68款空气压缩机·火焰处理机·UV光固机·金属蚀刻机·拉网机·晒版机·网版清洗机·烤箱/局炉·喷油柜·塑胶喷砂机·钢板研磨机·抛光机·制移印头设备·磨胶刮机·凉干架·油墨粘度器·调油墨机·打浆机·商标印刷机·气染印·水披覆纸印设备·热/水印纸印刷机·分切机·输送带设备/烫画机 5代理销售::进口油墨--德国Marabu马来宝;法国 Dubuit杜比;英国Apollo阿波罗;英国Coates高氏等;法国T3、瑞士Sefar赛发网纱;法国watts威特斯、胶刮等。

This paper infer to the "Debt shifting share"s legal issues is the no-determine of conimercial company"s legal position, To the problem, the penne suggest: determine Debt shifting share"s legal position, stipulate "capital charge company special law"and legal banner, perfect and attach importance to the company"s charge rules so perfect legal averment and circulation As a policy "debt shifting share " there is no existing experience to learn from."

针对这些问题,笔者相应地提出了一些对策建议,即明确&债股&三方的法律地位,制定&资产管理公司特别法&,建立法律约束机制,重视和完善公司治理结构等方面,从而完善法律环境,解决&债股&的法律问题。作为政策性&债股&,尚无现成经验可借鉴。商业银行&债股&是盘活银行不良资产,促进国有企业换机制的一项重大举措。

The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.

通过双向凝胶电泳分别分离TGIF染细胞和PcDNA3.1空白质粒染细胞的总蛋白质,测得两组细胞的蛋白质斑点数分别为760±41和680±38个;位置重复性分析表明TGIF染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm;PcDNA3.1空白质粒染细胞在IEF方向上的平均偏差为0.843±0.115mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。

operation process and arithmetic of the two-position gyro-based north seeker are introduced.

文章介绍了二位置陀螺寻北仪工作过程及算法原理,它采用陀螺仪在相差180°的两点上采样相互对消陀螺常值漂移的方法,提高寻北精度;其突出特点是结构简单,实现方便,精度较好;为了提高寻北精度和缩短寻北时间,文章找出误差源中的位误差,并分析了位误差对寻北误差的影响,推导了误差公式,得出位误差引起的寻北误差是一个常量的结论,同时提出了位误差的测量与补偿方法。

This article introduces the characteristics of color laser transfer printing technology and its procedures.

详细介绍了彩色激光印技术的工艺特点、流程、印纸的分类及激光印技术的应用前景,并与传统的网版印刷、喷墨/热升华印工艺进行了比较。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。