蹄

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

For every beast which has a divided hoof but does not have its hoof split in two or chew the cud is unclean to you; everyone who touches them shall be unclean.

11：26 凡走兽分蹄，但蹄不裂成两瓣，也不反刍的，对你们是不洁净的；凡触着它们的就不洁净。

For every beast which has a divided hoof but does not have its hoof split in two or chew the cud is unclean to you; everyone who touches them shall be unclean.

11：26 凡走兽分蹄，但蹄不裂成两瓣，也不反刍的，对你们是不洁净的；凡触着牠们的就不洁净。

And the pig, for though it has a divided hoof, that is, it has its hoof split in two, it does not chew the cud; it is unclean to you.

11：7 猪，因为分蹄，就是蹄裂两瓣，却不反刍，对你们就不洁净。

But you shall not eat any of the following that only chew the cud or only have hoofs: the camel, which indeed chews the cud, but does not have hoofs and is therefore unclean for you

但在反刍或有偶蹄的走兽中，你们不可吃的是骆驼，因为骆驼虽反刍，但偶蹄无趾，对你们仍是不洁的

But of them that chew the cud, but divide not the hoof, you shall not eat, such as the camel, the hare, and the cherogril: because they chew the cud, but divide not the hoof, they shall be unclean to you.

但是在反刍或有偶蹄分趾的走兽中，你们不可吃的：有骆驼、兔子和岩狸，因为它们虽反刍，蹄却不分趾，对你们仍是不洁的。

However these you shall not eat: of those that only chew the cud or of those that only have a divided hoof: the camel, for though it chews the cud, it does not have a divided hoof; it is unclean to you.

11：4 但那反刍或分蹄之中不可吃的乃是这些：骆驼，因为反刍却不分蹄，对你们就不洁净

However, of those that chew the cud or that have a split hoof completely divided you may not eat the camel, the rabbit or the coney.

但那些倒嚼或是分蹄之中不可吃的乃是骆驼，兔子，沙番因为是倒嚼不分蹄，就与你们不洁净。

Nevertheless these shall ye not eat of them that chew the cud, or of them that divide the hoof: as the camel, because he cheweth the cud, but divideth not the hoof; he is unclean unto you.

11：4 但那倒嚼或分蹄之中不可吃的乃是骆驼，因为倒嚼不分蹄，就与你们不洁净。

Nevertheless, among those that chew the cud or part the hoof, you shall not eat these: The camel, because it chews the cud but does not part the hoof, is unclean to you.

但那倒嚼或分蹄之中不可吃的乃是：骆驼因为倒嚼不分蹄，就与你们不洁净

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了