蹄

Camel foot type also known as the corpus sub-orders, although the characteristics of a ruminant, but their evolutionary history, already with the other separation of cloven-hoofed ruminants.

骆驼类亦称胼足亚目，虽然也具反刍的特性，但从其演化历史看，早已与其他反刍偶蹄类分开。

While intoxicated Shiqiu of diethylene glycol in the fragrance of the wine, wine aroma of the entire tribe has been diffuse, all men are ethnic reprimanded Shiqiu of Lisheng, Shiqiu of Nuqichongtian, God took his bow killing men struggling Family, men who fled the family fled to the most respected people - Yilun home, then at home Ailun heard outside million cloven-hoofed Ben riding and panic tapping for help, he even Xiangdoumeixiang, went straight to open the door out , said that at that moment, pull over arch Shiqiu of the moment to shoot, it hit in the heart of the Yilun, kind-hearted and innocent Yilun friends sacrificed their own lives.

正当赫丘力沉醉在酒的芬芳甘醇之际，酒的香气早已弥漫了整个部落，所有人马族都厉声斥责赫丘力，赫丘力怒气冲天，拿着他的神弓奋力追杀人马族，人马族们仓皇的逃至最受人尊敬的族人--奇伦家中，这时奇伦在家中听见了屋外万蹄奔踏及惊慌的求救声，他连想都没想，开门直奔出去，说时迟那时快，赫丘力拉满弓瞬间射出去，竟然射中了奇伦的心脏，善良无辜的奇伦为朋友牺牲了自己的生命。

Our company's manufaturing equipments include: 2 off automatic DISA 2013 vertical flaskless molding line, 1 off DISA2032 Boxless Moulding Line, 4 off five-toned environmental furnace cupola, 4 off PILLAR electric furnaces, several CK6142 numerical controlled machines, annular track casting cluster, shot blasting system, indirect duplex system with electric furnace and automatic sand preparation system, etc.

公司始建于是1997年，占地面积47000㎡，建筑面积18000㎡，职工400余名；可以生产灰铁150—300和球铁400--800的产品，主要有刹车盘、轮毂、刹车毂、制动钳体、支架、转向节、制动蹄铁、差减壳、飞轮、轮壳、发动机轴承盖、离合器压盘等的汽车配件，生产加工城市建设用下水井经箅子，井盖板等灰铁球铁铸造件，同时也根据客户的来图来样生产加工灰铁和球墨铸铁产品。

Its work principle is a cam to order about to make to brake hoof to open compressing tightly to make to brake drum making the car wheel system move thus.

它的工作原理是凸轮驱使制动蹄张开，压紧制动规片从而使车轮制动。

The rear brakes are "drum" type, wherein internal expanding brake shoe assemblies are forced against the machined surface of a rotating drum at each wheel to slow and stop the car.

汽车的后制动是鼓式制动，内部的外胀式制动蹄片总成和车轮转筒的机械加工面摩擦使汽车减速或停止。

The breeze in your face and pulling at your hair, the sun beats down, and all you can hear, bedsides the sound of yourself laughing and screaming with joy, is the sound of hooves pounding down on hard soil.

轻拂在你的脸和拉扯你的头发，太阳拍下来，和所有你能听到的话，病床边的声音，自己开怀大笑和尖叫与欢乐，是健全的蹄冲击，对硬土。

My song will please the LORD more than oxen, more than bullocks with horns and hooves

这将使上主满心欢愉，胜于蹄角具全的牛犊。

The monoclonal cells were selected using cloning rings. The expression of capsid protein was detected by indirect immunofluorescence and sandwich-ELISA. The empty capsids of FMDV were observed under electron microscope.

经间接免疫荧光和酶联免疫吸附测定（Enzyme-linked immunosorbent assay，ELISA）方法检测MDBK细胞中衣壳蛋白的表达，并在电镜下观察口蹄疫病毒空衣壳。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。