距离标记

Mapping of microsatellite markers in two wheat crosses segregating for Pm23 and Pm4b, respectively, in combination with the reported mapping of Pm4a, indicated that the three genes were all linked to the marker Xgwm356 with a distance of 3-5 cM. Allelism between Pm4b and Pm23 was then confirmed, when the progenies of a cross between VPM1 (Pm4b) and Line 81-7241, were shown to be all resistant to a B.

利用获得的8个标记对Pm4b进行遗传作图，结果表明，4个标记与Pm4b连锁(Xgwm356、Xgdm93、Xbar76和Xbarc122)。3个抗白粉病基因(Pm4a、Pm4b和Pm23)均与标记Xgwm356连锁，遗传距离为3-5cM，等位性测验证明Pm23与Pm4b为等位基因。

Genetic analysis were carried out to identify powdery mildew and strip rust resistance genes in the F2 of Am6-4 amphiploid and wheat variety Jinan17. Results showed that resistances to powdery mildew and stripe rust were controlled by a single dominant gene respectively. 124 SSR primers from genome D were used for marker analysis, marker Xgwm98 150(150为下标) from the chromosome 6D was found to be linked to the new powdery mildew resistance gene with a linkage distance 20.42 cM; A special DNA band was amplified by primere xgwm33 in resistant stripe rust plants, resistance gene for stripe rust was localized on chromosome 1D, and the genetic distance between resistance gene and marker is 8.0 cM.

利用Am6-4与济南17F2分离群体进行白粉病和条锈病抗性基因的遗传分析结果证明，Am6-4中的抗白粉病和抗条锈病基因均为单显性基因；以124对D基因组SSR引物进行标记分析，引物Xgwm98在抗白粉病DNA池和单株中能扩增出特异标记带，标记与抗白粉病基因间的遗传距离为20.42cM，并将抗白粉病基因定位于6D染色体；引物Xgwm33能在抗条锈病DNA池和单株中扩增出特异标记带，标记与抗条锈病基因间的遗传距离为8.0cM，并将抗条锈病基因定位于1D染色体。

One hundred and eighty three pairs of SSR markers were selected to construct a genetic linkage map, covering 1 762.2 cM and on the average distance of 9.63 cM between the markers.

在两种环境条件下种植以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建的259个F2：3家系群体，采用SSR标记构建了包含183个标记的玉米遗传连锁图谱，覆盖玉米基因组1 762.2 cM，标记间平均距离为9.6 cM。

Two hundred fifty-nine F2:3 families, developed from the cross between a dent corn inbred Dan232 and a popcorn inbred N04 were evaluated for its starch content in a replicated experiments under two environments. 183 pairs SSR were selected to construct a genetic linkage map with the genetic distance of 1 762.2 cM and on average 9.6 cM every marker.

在两种环境条件下种植以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建的259个F2∶3家系群体，采用SSR标记构建了包含183个标记的爆裂玉米遗传连锁图谱，覆盖玉米基因组1 762.2 cM，标记间平均距离为9.6 cM。

In present research it would provide the preliminary basis for marker assisted selection in cucumber breeding program and gene tagging of cucumber parthenocarpy.

遗传连锁分析表明，该标记与单性结实基因连锁距离为18.9cM，为黄瓜单性结实基因的分子标记筛选和分子标记辅助育种奠定了初步基础。

Methods: In control group, the studied model was X-rayed with the central mark of scale penetrated vertically by the centerline of X-ray-tube and both ends of the scale included in the radiation field.

对照组：X线球管中心线对模型1标尺的中点标记进行垂直摄影，照射野包含标尺的两侧标记，摄影距离120cm，物－台距离3.5cm。

Ofeffective 1st branches, length of main inflorenscence, effective siliques of maininflorenscence, density of main inflorenscence, length of silique, seed per sillique,1000 seed weight, total effective siliques per plant had significant positive correlationwith yield per plant. Polymorphisms between zhongshuang No.4 and H228 was examined by SSR primers. 152 primers Showed polymorphisms, and a linkage map containing 123markers, including 18 linkage groups was constructed. The total genetic distance ofthe map was 3483.1cM, and the average distance of two adjacent markers was28.32cM.

选用SSR引物对中双4号和H228间进行多态性检测，有152对引物在双亲间表现多态性，用这些引物分别对142个株系进行检测，构建了含有123个SSR分子标记、18个连锁群的连锁遗传图，总图距为3483.1cM，标记间平均距离为28.32cM，各标记在18个连锁群上的分布很不均匀，其中，第1、12、13、17连锁群上分布标记较多，其它连锁群上分布标记较少。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Moreover, the high frequency radio can also be used in the optical label switching. The subcarrier multiplexing optical label and optical carrier suppression and separation optical label are based on modulating lightwave by radio, which were studied much and maybe have a good future.

另外，在光标记交换领域，利用高频的无线信号调制光波实现的副载波光标记和光载波抑制光标记现已有一些研究，这两种标记实现方案有很好的应用前景，但还有一些问题需要深入研究，本论文对SCM光标记信号的传输距离受限问题和OCSS光标记分组的全光波长变换问题进行了深入研究，并得到了一些有价值的结论。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。