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超速

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He was ticketed for speeding.

他因超速而被开了罚单。

The main results obtained were summarized as follows:Setcreasea purpurea Boom.has the ability to hyperaccumulate Cu.When the supply of Cu was 1000μmol·L~(-1),the Cu concentration in shoots and roots was up to 1105 and 1210 mg·kg~(-1)DW,respectively.Copper uptake and translocating efficiency of Setcreasea purpurea Boom.depended on the Cu concentration in nutrient solution.

为试验材料,综合运用溶液培养法、相关酶分析方法、ICP-AES元素测定技术、差速和超速离心技术以及分子生物学研究技术等从几个不同方面研究了该植物对Cu的超积累机理,取得了如下的结果。

In this paper, purification of growth hormone receptor from ovine liver and identification of its biochemical character have been done.Firstly, by homogenizing, triturating, high-speed centrifuging, hypervelocity centrifuging and sucrose density grade centrifuging cell membrane of ovine liver was separated and its electron microscope slice wasprotein was disL~olved with TritonX-100,amL-thebinding activity between cell membrane or membrane protein and ~I-hGH using RRAwas measured, perfect saturated curve wasgained. Thirdly, specific binding of GILR protein was measured ,by SDSPAGE, its molecular weight is about 1 19KD.

本研究采取新鲜羯羊肝脏,匀浆、研磨,经高速离心,超速离心和蔗糖密度梯度离心,分离肝脏质膜,得到肝脏质膜的电镜切片:其次,用去垢剂TritonX-100增溶膜蛋白,利用放射受体分析法,测定细胞膜及膜蛋白与~(125)I-hGH的结合活性,均得到理想的饱和曲线;再次,将两点结合试验法与凝胶层析法结合检测到特异结合物,然后,经SDS-PAGE电泳分析,纯化后的样品呈现一条带,与标准分子量比较,得出分子量为119KD。

The results showed that plenty of the purified DPV virions were obtained after the cell lysate was condensed by 5-fold by ultrafiltration,and the concentrated DPV fluid was ultracentrifuged with 300g/L sucrose cushion method and 300-600g/kg sucrose density gradient centrifugation methods.

结果显示,用切向超滤法将病毒培养物浓缩为原体积的1/5后,再采用300g/L蔗糖垫底超速离心和300~600g/kg蔗糖密度梯度离心的方法进行纯化,可获得大量的纯化病毒粒子。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2/6/7病毒样颗粒,待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解,培养基上清和细胞裂解液样品两次40%蔗糖垫93000g超速离心,沉淀样品经SDS-PAGE胶分离,用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性,电镜检测重组病毒样颗粒的形态结构。

HDL reference, in which 1μg apoAI was thought of as one activity unit, was prepared by ultracentrifuging.

测定时,以超速离心制备的HDL参考品为标准,按每1μg apoAI为1个HDL活性单位,由参考品的光吸收值和相应的活性单位数,求出HDL生物活性标准曲线的双对数回归方程,并由样品的光吸收值计算HDL制剂的生物活性。

Low Speed Large Capacity Centrifuge LD5-10B. The Model LD5-10B is computer controlled and driven by brushless motor, having functions of digital displaying, multi-function pre-setting, braking, warning for over speed and speed out of control, and uncapping protection.

无刷电机,微电脑控制,旋转编码器调节,全不锈钢旋转件,能编辑和存储9种程序,自动计算显示离心力,大屏幕数字显示,电控门锁,快速启动,快速停机,具有超速、失速等多种报警和保护功能。

Low Speed Large Capacity Centrifuge LD5-10. The Model LD5-10 is computer controlled and driven by brushless motor, having functions of digital displaying, multi-function pre-setting, braking, warning for over speed and speed out of control, and uncapping protection.

无刷电机,微电脑控制,旋转编码器调节,全不锈钢旋转件,能编辑和存储9种程序,自动计算显示离心力,大屏幕数字显示,电控门锁,快速启动,快速停机,具有超速、失速等多种报警和保护功能。

The 450/80t casting hoist crane host lifts the organization disposition emergency brake goal is prevented after the work brake transmission chain appears the question or the host lift the system overspeed,the emergency brake can the rapid ground floodgate,thus avoids having the molten steel pot effectively to crash and so on significant acci.

450/80t 铸造起重机主起升机构配置紧急制动器目的是防止当工作制动器之后的传动链出现问题或主起升系统超速时,紧急制动器能迅速地上闸,从而有效避免发生钢水罐坠落等重大事故的发生。

Precisely at the moment in history when we need to shift our system of food production into overdrive, our agricultural engine is breaking down, he says.

在需要粮食生产超速发展的这一历史时刻,我们的农业引擎却恰好发生了故障,他说。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

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