超生物学

The relative conductivity of leaves, ultrastructural changes of mesophyllous cells under heat acclimation and heat stress, the differences in gene expression of leaves under hest shock can be used as physiologic, cytologic and molecular biologic indexes respectively for evaluating heat tolerance.

在热锻炼和热胁迫过程中叶片相对电导率、叶肉细胞超微结构的变化，以及热击条件下叶片基因表达差异，可分别作为耐热性评价的生理学、细胞学和分子生物学指标。

TGF-β s are multifunctional cytokines with a broad range of biological activities. TGF-β s belong to a family of related proteins, which demonstrate various degrees of amino acid sequence homology; this protein family is called the TGF-β superfamily. Other members of this family include the bone morphogenetic proteins, inhibin, activin and Mullerian substance.

TGF-β类生长因子是一类具有广泛生物学活性和重要临床应用价值的细胞因子，包括多种蛋白因子，它们在氨基酸结构上具有不同程度的同源性，共同构成了TGF-β超家族，包括BMPs、活化素、抑制素和缪勒氏物质等。

Methods BMSCs isolated from rabbit iliac bone marrow were culture-expanded in vitro. The injectable tissue-engineered bone constructed from autologous PRP, FG, and BMSCs was cultured in vitro, and its biological characteristics were observed including the time of gel formation, histological features, seed cell survival and microscopic structures. Results The constructed injectable tissue-engineered bone began gel formation within 20 to 30 s, and after a week-long culture, the gelatine began to degrade, and numerous well viable fusiform cells could be seen to adhere to the bottom of the Petil dish, Scanning electron microscopy identified globular and olivary cells embedded in the fibrin glue, and numerous small particles could be seen around of the cells.

从兔髂骨处抽取骨髓体外培养BMSCs并诱导向成骨细胞分化，抽取兔自体动脉血提取PRP，以FG、BMSCs、PRP共同构建可注射型组织下程骨并体外培养观察其生物学特性如凝胶形成时间，组织学特点、细胞存活情况及其超微结构特征等结果构建的可注射型组织工程骨可在短时间内形成凝胶，体外培养1周时其中细胞生长良好，电镜下见纤维蛋白网格结构致密，种子细胞及血小板颗粒分布广泛。

Having long lived in rigor habitats,two extreme xerophytes Reaumuria soongorica and Salsola passerina formed special biological features and physio-ecological adaptations.

超旱生植物红砂与珍珠长期生活在严酷的生境中，形成了独特的生物学特性和生理生态适应性。

We used transmission electron microscopy to study the ultrastructural changes in egg cell, zygote and two-celled proembryo of Beta vulgaris to provide information on the reproductive biology of angiosperms and related research into sugar beet.

为丰富被子植物生殖生物学资料，并为甜菜相关研究提供参考，应用透射电镜技术研究栽培甜菜卵细胞、合子和二细胞原胚的超微结构特征。

It has been ratified by nativemedicine supervisory bureau and in the third stage ofclinical obserbison.The study took acute myocardialischemia rabbit as animal model and long Xin Luo capsuleas control medicine.We proke into the mechanism of actionof Yi Xin capsule on preventing and curing myocardialischemia.

本研究以急性心肌缺血家兔作为动物模型，以通心络胶囊作为对照药，从血管生物学效应、病理形态学及超微结构等方面，探讨了益心胶囊防治心肌缺血的确切作用机制。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Results The survival area and quality of avulsed flap in the experimental group were superior to that of control group after 15 days of operation, and the hemorheological items were markedly changed at 5 days after operation. Conclusion The better flap survival after orthotopic transplantation in avulsion injury can be improved by intravascular low level He-Ne laser irradiation through changed superoxide dismutase activity and hemorheological items in optimal irradiation intensity.

结果 术后15天治疗组撕脱瓣的成活面积、成活质量均明显优于对照组P结论低能量He-Ne激光血管内照射的生物学效应存在最佳的照射强度总量，在最佳的照射强度条件下，通过改变细胞内超氧化物歧化酶活性及血液流变学参数促进原位回植术后的撕脱瓣良好成活。

By now, there is seldom report on the Heilongjiangwood frog which hibernates at low temperature condition. Our study mainly analyze theadaptation changes of liver proteins, SOD, LDH, G6PDH and EST activities of Heilongjiangwood frog under low temperature.

本文旨在研究低温条件下黑龙江林蛙肝脏蛋白质、超氧化物歧化酶、乳酸脱氢酶、葡萄糖6-磷酸脱氢酶(G6PDH)及酯酶的适应性变化，以探讨黑龙江林蛙冬眠期低温耐受的机制，为两栖类低温生物学和比较生理学的深入研究提供理论参考和实验依据。

A common sense interpretation of the facts suggests that a superintellect has monkeyed with physics, as well as chemistry and biology, and that there are no blind forces worth speaking about in nature.

对这些事实的一个常识解读暗示，一个超智者作弄了物理、化学和生物学，自然界里并没有什么盲目的力量可言。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。