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Objective To observe effects of Tuber Fleeceflower Root serum on activity of SOD, the expression of c-myc mRNA and the activity of telomerase of human fetal lung diploid fibroblasts (2BS).

作者:李朝敢,韦星,农嵩,韦耀东,张树球目的观察含何首乌血清对人胚肺二倍体成纤维细胞(2BS)超氧化物歧化酶活性、c-myc mRNA表达及端粒活性的影响

The results showed that at the beginning stage of being attacked by Chinesis white pine beetle and their symbiotic blue-stain fungus, PAL, PPO, SOD activities and MDA content significantly increased in the phloem of P. armandi, and decreased with the increase of the number of D. armandi and the attacking on the xylem and phloem of P. armandi from blue-stain fungus, then reached the minimum level in withering stage of P. armandi. But the contents of nutrition materials in the phloem, such as water, protein, glucide, fat, amylum and so on, declined with the infestation of D. armandi and symbiotic fungus. In summary, the resistance of physiology and biochemistry in the phloem of P.

结果表明,在华山松大小蠹和共生蓝变真菌危害初期,华山松韧皮部内的苯丙氨酸解氨酶、多酚氧化酶、超氧化物歧化酶活性均显著提高,丙二醛含量明显增加;但随着华山松大小蠹种群数量的增加和蓝变真菌对华山松韧皮部和木质部组织危害的加剧,韧皮部组织内的PAL、PPO和SOD活性逐渐降低,在枯立木阶段降至最低;与此同时,华山松韧皮部内的水分及蛋白质、糖类、粗脂肪、淀粉等营养物质含量,则随华山松大小蠹和共生蓝变真菌危害的加剧而不断减少。

The experiments were conducted to find out the effects of calcium, copper, zinc, manganese ions, SDS and EDTA (ethylene diamine tetraacetic acid) on the superoxide dismutase in muscle of kuruma shrimp Penaeus japonicus, prawn Procambarus clarkii and Bacific white shrimp Litopenaeus vannamei and the differences in SOD in muscle, gill and hepatopancreas.

测定了Ca(上标 2+)、Cu(上标 2+)、Zn(上标 2+)、Mn(上标 2+)及SDS、EDTA不同浓度对日本对虾、克氏原鳌虾、凡纳滨对虾肌肉组织中超氧化物歧化酶活性的影响及其肌肉、鳃、肝胰腺组织中SOD活性的差异,并利用电泳技术研究了组织同工酶。

Armigera larvae. For the larve under the 750μl/L CO2, their free fatty acid, SOD, TChE and AMS significantly increased, whereas their protein, total amino acid and GSHPX significant decreased.

与对照比较,750μl/L CO2饲养下棉铃虫幼虫体内蛋白质和总氨基酸含量分别下降了14.16%(p.01)和28.40%(p.01);超氧化物歧化酶、乙酰胆碱酯酶和淀粉酶的活性分别增加了26.43%、9.12%和40.17%,而谷胱甘肽过氧化物酶的活性则下降了20.25%(p.01)。

In this study, the malondialdehyde content, the glutathione peroxidase activity, and the superoxide dismutase activity in black porgy Sparus macrocephalus were determined after the acute exposure to TBTCl at the concentration of 4 and 20 μg·L-1. Results showed that TBTClinhibited the SOD activity in S.macrocephalus gill slightly , but there was an obvious promotion on the SOD activity in S.macrocephalus liver.

摘 要:观察和比较了黑鲷 Sparus macrocephalus 在不同浓度的氯化三丁基锡(tri-n-butyltin chloride,TBTCl)溶液中暴露后,其鳃和肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)活性的变化情况。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion(O2-), which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白,其主要特征为依赖刺激而活化,机制为复合体的胞浆因子磷酸化并转位至膜,在这里与细胞色素酶相关联,组装完全的复合体在功能上作为一个电子转运体系,从胞浆NADPH转移电子给分子氧形成超氧阴离子(O2-),伴随着接下来的活性物质产生,也叫呼吸爆发,发挥抗菌和抗细胞毒素的活性。

It is found that the low dielectric constant inhibits the disproportion of pyrogallic, but it can be settled by increasing the pH or the water content of the reverse microemulsion to obtain a stable generation of the substrate. Then the activity of SOD is determined in such a system and a bell -shaped curve of the activity vs. W〓 is obtained although only subactivity is found. The reasons can be attributed to the low dielectric constant and the attraction of CTA〓.

的介电常数的降低对邻苯三酚的自氧化有抑制作用,因此实验中通过提高CTAB微乳液的表观pH或增大水化度即W〓,最终得到了一定浓度的底物超氧阴离子O〓〓·,保证了SOD活性的测定,并得到了SOD酶活性随含水量变化的&钟形&曲线。

The results showed that memberane permeability increased,POD and SOD activity and soluble protein content decreased in the Raphanus L.seedlings under 300 mg/L lead stress.

结果表明:在300 mg/L铅胁迫浓度下萝卜芽苗菜的膜透性明显增大,过氧化物酶活性、超氧化物歧化酶活性、可溶性蛋白含量明显下降。

Memberane permeability firstly decreased and then increased,POD and SOD activity and soluble protein content firstly increased and then decreased after Raphanus L.seedlings were strayed by different concen...

随着外施EDTA浓度的增加,萝卜芽苗菜的膜透性先下降后升高,过氧化物酶活性、超氧化物歧化酶活性、可溶性蛋白含量先升后降。

Results:Sorbaria sorbifolia extract significantly increased the activity of SOD in brain tissue, decreased the content MDA, inhibit the MAO activity. It was observed under microscope that Sorbaria sorbifolia could retrieve the degeneration of mitochondrion.

结果:珍珠梅提取物可提高D半乳糖所致大鼠脑组织SOD活性、降低MDA含量,抑制MAO活性;电子显微镜观察到珍珠梅提取物可以减轻D半乳糖导致的线粒体等超微结构的改变。

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