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A modified technique was developed to assay superoxide dismutase activity by combining polyacrylamide gel electrophoresis and densitometry.

改良技术开发检测超氧化物歧化酶活性的结合凝胶电泳法和密度。

These parameters did not differ from each other in figleaf gourd"s two treatments. The root temperature of 14℃didn"t induce significant changes in the total respiration rate in both species but caused an increase of the alternative respiration pathway and a decrease of the cytochrome respiration pathway, and more dead cells in root tips in cold-sensitive cucumber roots.

低温条件下,黄瓜的生长明显差于黑籽南瓜,同时根系表现出更高的超氧化物阴离子O_2~(·-产生速率、过氧化氢(H_2O_2)与膜脂过氧化产物丙二醛含量以及抗氧化酶活性。14℃根温并未引起两种作物根系的总呼吸速率发生变化,但却引起黄瓜根系的交替途径呼吸速率与细胞色素途径呼吸速率的显著变化,表现为低温下Valt的上调而Vcyt的下降。

ATP content was detected by high performance liquid chromatogram. Mitochondrial membrane potential was measured by rhodamine123 (Rh123) staining and photofluorography. The activity of superoxide dismutase was observed using a SOD kit. The expression of heme oxygenase-1 (HO-1) was evaluated by Western blotting.

应用高效液相色谱法检测细胞内ATP的含量;罗丹明123(Rh123)染色荧光显微镜照相检测线粒体膜电位;超氧化物歧化酶检测试剂盒检测SOD活性;免疫印迹法检测血红素氧合酶-1(HO-1)的表达。

Methods Male SD rats were given drinking water containing manganese chloride (MnCl24H2O 0.5g/L and 5.0g/L respectively) for 60 days. At the end of experiment, all the rats of the manganese-exposed groups and control group were decapitated after anaesthesia with 2% sodium phenobarbitone intraperitoneally, the brains were removed and activities of alanine aminotransferase, aspartate aminotransferase, alkaline phosphate, cholinestarase, monoamine oxidase, superoxide dismutase, nitric oxide synthase, and levels of glutathione, maleic dialdehyde, and proteins were biochemically analyzed.

大鼠经饮水连续染锰(MnCl24H2O 0.5g/L和5.0g/L)60d后,应用荧光光度比色法测定新鲜脑组织匀浆的谷丙转氨酶、谷草转氨酶、碱性磷酸酶、胆碱酯酶、单胺氧化酶、氧化亚氮合酶、超氧化物歧化酶活性和谷胱甘肽、丙二醛、蛋白质含量,并设生理盐水对照组。

Methods the models of epilepsy were made by intraperitoneal injection of pentylenetetrazol and the contents of no,mda and sod of all the epileptic hippocampus were assayed and dealt with all data by statistical analysis.

健康sd大鼠38只,分5组,采用戊四氮腹腔注射制作癫痫模型,测定各组大鼠海马一氧化氮、丙二醛的含量和超氧化物岐化酶的活性。

The results showed that the electrolytic leakage and the content of MDA in leaves treated with cold acclimation were lower than those of non-treated, the contents of proline and the activities of SOD and 5'-nucleotidase kept higher levels compared with those of controls.

通过对其相对电导率、丙二醛和脯氨酸含量及5'-核苷酸酶、过氧化物歧化酶和超氧化物歧化酶活性的测定,研究了低温锻炼对菊花抗寒性的诱导。

The polysaccharide can inhibit the growth of Escherichia coli and Staphyloccocus aureus, and the scavenging effects of polysaccharide on the superoxide anion radical and nitroso were significant. It could be concluded that the polysaccharide displayed obvious bioactivities.

该多糖对埃希氏大肠杆菌和金黄色葡萄球菌均有抑制作用且对超氧阴离子自由基和亚硝基有较强的清除作用,说明该多糖具有明显的生物活性。

The apple polyphenol extracts had stronger reducing capacity, antioxidant activity on lipid-peroxidation, and scavenging effects on hydroxyl radical, superoxide anion radical and nitroso than ascorbic acid at the same concentration.

按照以上工艺制备的苹果多酚提取物具有较强的还原能力和抗脂质过氧化活性,并对羟自山基、超氧阴离子自由基及亚硝基具有很强的清除作用,且优于同浓度的维生素C。

8Ps for native and NaBH〓 chemically modified reaction centers, and the transfer process from H〓 to the final electron acceptor Q〓 occurs in about hundreds of picoseconds. On the other hand, the energy transfer from excited B was obviously slowed down in the pheophytin-exchanged RC. By considering the change of free energy of ion pair P〓Phe〓, it could be expected there might exist alternative pathway of charge separation and electron transfer in pigment-exchanged reaction center. In addition, the influence of acid environment on the ultrafast dynamics of reaction center was also experimentally observed, which shows an evident difference from that of in native RC, and further investigation should be carried out to elucidate the origination.

在色素置换后的反应中心中,以B为起点的能量传递较天然RC的情况明显减慢,约在450fs左右,考虑到置换后的反应中心中P的自由能有所提高以及其仍然保持的光化学活性,这一较慢的能量传递也可能预示着还存在着其它的能量传递通道;另外,还对酸性处理的反应中心中的超快光动力学过程进行了尝试性的研究,发现由于色素分子的聚集以及蛋白环境的改变,BChl分子的激发态动力学过程有着明显不同于天然反应中心的情况。

AIM: To investigate the influence of bradykinin to plasma or myocardial malondialdehyde level or superoxide dismutase activity in rabbits with acute myocardial infarction, and to study the protection mechanism of BK to myocardium.

目的:观察缓激肽对血浆、组织超氧化物歧化酶活性的影响,探讨BK对心肌保护作用的机制。

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