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Methods Fe2+VitCinduced lipid peroxidation damage models were established by rat liver microsomes extraction. The expressions of malondialdehyde and superoxide dismutase were tested.

通过大鼠肝微粒体的提取,制备Fe2+VitC系统诱导的脂质过氧化损伤模型,检测车前子多糖对丙二醛的抑制作用以及对超氧化物歧化酶活性的影响,观察车前子多糖的抗脂质过氧化作用。

Then we detected the mitogen activity and TCRⅤβ specificity of SED with human PBMC and mice plenocytes.

获得了纯度较高,并具有超抗原活性的SED,为构建SED突变体和后续的功能研究奠定了良好的基础。

This dissertation aims at finding anticancer and antibacterial polynuclear complexes targeting DNA with high activity and low toxicity. Sixteen compounds were synthesized, and characterized by means of X-ray single crystal diffraction, elemental analysis, molar conductivity and IR, and the influencing factors on structures of the compounds have been investigated. The actions of these compounds to DNA were also investigated by spectral, electrochemical and hydromechanical methods.

本论文以DNA为靶点,以寻找新结构类型的高效、低毒的抗癌、抗菌多核配合物为目标,从分子设计出发,共合成得到了16个化合物的单晶结构,对其进行了结构表征,探讨了结构影响因素,并研究了其超分子结构;采用紫外-可见吸收光谱法、荧光光谱法、电化学和粘度法,从分子水平上研究了所得配合物与DNA的相互作用;在细胞水平上,研究了草酰胺桥联双核配合物的抗菌和抗肿瘤活性。

Objective To investigate the biological characteristics of superparamagnetic iron oxideslabeled myoblasts in vitro.

目的 研究超顺磁性氧化铁纳米粒子标记成肌细胞方法及其对成肌细胞活性的影响。

The left anterior de scending branches were ligated in anesthetic dogs to establish acute myocardial infarction models. The activities of AST, CK and LDH in serum were detected and the changes of ultrastructure of myocardium were observed.

采用麻醉开胸结扎犬的冠状动脉前降支制备急性心肌梗塞模型,取静脉血检测血清中天门冬氨酸转氨酶、磷酸肌酸激酶和乳酸脱氢酶活性;应用透射电子显微镜观察心肌细胞超微结构的改变。

Methods After injecting different doses of cephalosporins(100, 200, 300, 400μg) into vitreous body of rabbits, we studied the active change of retinal enzymes at different time and observed the histological and ultrastuctural changes of retinae.

方法以不同浓度的先锋霉素Ⅵ(100,200,250,300,400μg)行兔眼玻璃体腔内注射,用酶组织化学染色法测定注射后不同时间(1,3,7d)视网膜琥珀酸脱氢酶和乳酸脱氢酶活性的变化,并观察对视网膜组织结构及超微结构的影响。

Results The activity, recovery rate of activity and half-life of immobilized SOD were 333 U/g, 86.32% and 43.8 d respectively. After storage at room temperature for 5 d, the relative activity of SOD was still more than 80%. The optimal reaction temperature of immobilized SOD was 45℃, and the activity retentions after being used for the first and second times were 70.12% and 51.72% respectively. Both the optimal pH values for SOD before and after immobilization were 6, while the Km values were 0.16 and 0.18 mmol/L respectively.

结果 固定化超氧化物歧化酶活力为333U/g,酶活回收率为86.32%,半衰期为43.8d;固定化酶室温保存5d后,相对酶活力仍保持在80%以上,最适反应温度为45℃,使用一次后回收率为70.12%,重复使用两次后回收率为51.72%;固定化酶与溶液酶在pH6时活性最强,Km分别为0.18mmol/L和0.16mmol/L。

To sample the radicles and cotyledons respectively while the radicles was 1 cm long, the total soluble protein, the malondialdehyde content, the activites of superoxide dismutase, the catalase and the peroxide enzyme were determined.

确定供试种子批种子活力正常后,在萌发种于胚根长到1 cm时,分胚根和子叶两个部位取样测定其可溶性蛋白质含量、丙二醛含量及超氧化物歧化酶、过氧化氢酶和过氧化物酶活性等生理指标。

When the synovial line structure appeared around the silica gel bag, we observed the bursa figure, studied its histology and ultra structure, detected the activity of uridine diphosphoglucose dehydrogenase in synovial lining structure. At the same time, the expression of vascular cell adhesion molecule 1 (VCAM-1), CD68 and UDPGD mRNA in cells was detected, the character of liquid in subcutaneous synovial bursa was studied, and hyaluronic acid concentration of liquid was detected with ELISA. Secondly, a portion of peroneus longus tendon was taken from self-body of rabbit and it be placed into the artificial subcutaneous synovial bursa.

首先,在动物皮下手术埋置硅胶囊扩张器,经过不同时间充气和有规律的按摩刺激,使局部形成滑膜样包裹结构,从而构建人工皮下滑膜囊模型,通过大体观察、组织学结构、超微结构、免疫组化以及UDPGD功能性酶活性的检测、滑囊液HA浓度检测等指标,对滑膜囊结构和功能进行研究,探讨其形成的可能性。

Patients with hepatic cirrhosis and 10 healthy controls were studied. Plasma VIP and CCK were measured with radioimmunoassay and gallbladder emptying function was measured by ultrosonography with fat meal test. The plasma VIP and CCK of patients were all higher than that of controls.

用放免法测定肝硬化患者及对照者血浆血管活性肠肽和胆囊收缩素含量;用B超测定餐前、餐后胆囊容积及排空率。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。