超活性

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The results showed that mitochondria lipid peroxidation level significantly elevated, SOD activity and GSH content remarkedly reduced after exercise immediately.

发现运动后即刻心肌线粒体脂质过氧化水平显著升高，超氧化物岐化酶活性和还原型谷胱甘肽含量显著下降。

This paper summarized the development of metallocene catalyst and single-site center technology, and introduced the developing trends of polyethylene producing processes including bimodal PE production technology, responser new disposition technology and condensed and super-condensed technology.

概述了茂金属催化剂和单活性中心技术的发展，介绍了聚乙烯生产技术新的发展动向，如双峰PE生产技术、反应器新配置技术、冷凝态和超冷凝态技术。

Toxic effects of HgCl2 on Scenedesmus obliquus were studied in this paper. Algal growth, photosynthetic pigments content, super oxide dismutase activity, malondialdehyde content in the algal cells were measured in the exponential phase.

为探讨汞对藻类的毒性效应，以斜生栅藻为实验材料，检测了HgCl2对藻细胞数目、光合色素含量、超氧化物歧化酶活性、丙二醛含量等生化指标的影响。

Methods The rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

采用大鼠大脑中动脉缺血2 h/再灌注22 h模型，神经病学评分，脑梗塞范围及脑组织水含量变化，观察脑康灵胶囊抗脑缺血/再灌注损伤的效应；通过测定大鼠脑组织中一氧化氮合酶（nitric oxide synthase，NOS），超氧化物歧化酶（superoxide disumtase，SOD）活性及丙二醛（malondialdehyde，MDA）含量的变化以探讨药物作用的机制。

MethodsThe rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

方法采用大鼠大脑中动脉缺血2 h/再灌注22 h模型，神经病学评分，脑梗塞范围及脑组织水含量变化，观察脑康灵胶囊抗脑缺血/再灌注损伤的效应；通过测定大鼠脑组织中一氧化氮合酶（nitric oxide synthase，NOS），超氧化物歧化酶（superoxide disumtase，SOD）活性及丙二醛（malondialdehyde，MDA）含量的变化以探讨药物作用的机制。

Chlorophyll content, Soluble protein content, POD activity, CAT activity, SOD activity, and MDA content of Spirodela polyrhiza were measured, and transmission electron microscopy was observed.

主要测定了叶绿素含量、可溶性蛋白含量及抗氧化系统的几种酶，包括印POD、CAT、SOD的活性以及MDA含量的变化，并观察了叶绿体超微结构的变化。

The abilities to synthesize, protective enzymes and chloroplast structures of Spirodela polyrhiza were changed when this plant was expose to cadmium pollutant water.

研究表明，在短期镉胁迫下紫萍的合成能力、保护酶活性及叶绿体超微结构均发生变化。

0Using experimental materials of one-year old steckling from six hybrid tulip clones (NE04, NE22, NE23, NE25, NE38, NE78), the relative water content, permeability of plasma membrane, soluble protein and proline contents, activity of superoxide dismutase, and ascorbate peroxidase were analyzed under drought stress.

以6个杂交鹅掌楸无性系(NE04、NE22、NE23、NE25、NE38、NE78)的1年生扦插苗为试材，研究了干旱胁迫条件下各无性系叶片相对含水量、质膜相对透性、可溶性蛋白质和游离脯氨酸含量、超氧化物歧化酶及抗坏血酸过氧化物酶活性等的变化。

Thus, research on solid superacid has drawn more and more attention in industrial catalyst field.

采用溶胶-凝胶法制备了固体超强酸催化剂，考察它们在酯化反应中的催化活性。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。