超显微镜的

Characteristics and operating stages of these instruments or measuring machine. Scientific research tasks includes: the CMM may be used in studying CAD,CAM ,CIM and IE; AFM used in topography of fine manufactured surface, nano- material semi conductor, chemical or chemical Eng .etc.

科研任务涉及三坐标测量可用于 CAD、CAM、CIM及IE等领域研究；原子力显微镜的超精细加工表面轮廓测量，纳米材料（含半导体材料、气体、化学、化工材料）、生物样品等微表面分析测试。

The effects of β-sitosterol on morphology, microstructure, cell cycle and apoptosis were studied by phase-contrast microscope, electron microscope and flowcytometry, respectively.

采用倒置相差显微镜、扫描和透射电镜观察细胞形态学和超微结构的变化，流式细胞术检测β-谷甾醇对细胞周期和细胞凋亡的影响。

The ultramicroscope, the UV microscope and the luminescence microscope exemplify the inventive genius of those years and reflect three goals of microscopy, which have remained topical to date: Making ever smaller dimensions accessible to observation; observing living objects without damaging them; and finding methods to contrast the substances in such objects.

超显微镜，紫外显微镜、冷光显微镜的发明向我们展示了那个年代的天才智慧。同时也反映了显微术不断追求的三个目标：观察到更小尺度的物体；避免观察对活体的损伤；提高观察对象的图像反差。

With ultramicroscopy, it should be possible to accomplish the same in one hour.

通过超显微镜技术达到同样的目的在一小时内就能完成。

Dodt, ultramicroscopy has even a wider range of applications than confocal microscopy, and therefore, the impact will be enormous.

按照Dodt博士的说法，超显微镜技术与共聚焦显微镜技术相比具有更大的应用前景。

Ultramicroscopy after Siedentopf and Zsigmondy makes visible submicroscopic particles whose linear extension is below the microscope's resolution limit.

超显微镜可以看到亚显微颗粒的线性延伸，而这是普通显微镜的分辨率无法达到的。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

The ontogeny, phylogeny and ultrastruct of the glandular hairs of 5 varieties and 38 species in 26 genera of Labiatae are studied with the light microscopy, the scanning electron microscopy and the transmission electron microscopy in the paper.

本文利用光学显微镜、扫描电子显微镜和透射电子显微镜对唇形科26属38种和5变种的腺毛进行了个体发育、系统发育和超微结构的研究。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Objectives: To study the influence of the low intensive pulse magnetic field on ultrastructure of epidermal stem cells.

目的：应用原子力显微镜和透射电镜观察低强度电磁场对表皮干细胞超微结构的影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了