超显微镜

The thermal properties, mechanical properties of PI-MWNTs nanocomposite were analyzed by Thermogravimetric analysis, Universal Test Machine and Hardness Pencil Test. The morphology of PI-MWNTs was observed by scanning electron microscopy. The dispersion of MWNTs in matrix were observed by transmission electron microscopy. The electrical property of composite were obtained by Super Megohmmeter.

研究中使用傅立叶转换红外光谱仪来鉴定其化学结构，热重损失分析仪探讨材料的热稳定性质，万能试验机及铅笔硬度计观察其机械性质，扫描式电子显微镜与穿透式电子显微镜来观察材料的表面型态及奈米碳管於基材中的分散性，超绝缘仪量测材料的电性。

The thermal properties and mechanical properties were analyzed by Thermogravimetric analysis and Hardness Pencil Test and Universal Test Machine, respectively. The structure of prepared PI-MWNTs was characterized by Fourier transform infrared spectrometry. The morphology of PI-MWNTs were observed by scanning electron microscopy and transmission electron microscopy. The electrical conductivity was analyzed by Super Megohmmeter.

本研究中使用能量分散光谱仪来鉴定多壁奈米碳管纯化效果，热重损失分析仪、铅笔硬度计、万能试验机分别测定材料的热稳定性质和机械性质，傅立叶转换红外光谱仪来鉴定其化学结构，扫描式电子显微镜与穿透式电子显微镜来观察材料的剖面型态与奈米碳管和基材之间的分散性，超绝缘量测仪量测材料的电性。

Production and sales companies: particle detector strength, freeze-drying machine, snow ice machine, glass reactor, rotary evaporator, electronic balance, ultrasonic cleaning machines, ice making machine, industrial ice-making machine, constant temperature and humidity box, microscope several significant oil bath temperature pot / water bath pot, Ma fluoride furnace, low temperature cooling liquid circulating pump, circulating water-type multi-purpose vacuum pump, mold incubator, biochemical incubator, constant temperature incubator, vacuum oven, blast oven, super - net workbench, biosafety cabinets, magnetic stirrer, centrifuge, ultrasonic cell pulverizer, ultra-pure water machines, autoclaves, laboratory various beaker, Bio-Rad power supply, test-bed, constant temperature circulator, low temperature cycle at high pump, pesticide Tachometer, UV analyzers, liquid machine, PCR device, liquid chromatography, weather chromatograph, total acidity / PH, the spectrophotometer, ultra-low temperature refrigerators, constant-current pumps, rotary vane type vacuum pump, auto parts traps, low-temperature thermostat bath; and other products.

公司生产销售：颗粒强度测定仪，冷冻干燥机，雪花制冰机，玻璃反应釜，旋转蒸发器，电子天平，超声波清洗机，冰块制冰机，工业制冰机，恒温恒湿箱，显微镜，数显恒温油浴锅/水浴锅，马氟炉，低温冷却液循环泵，循环水式多用真空泵，霉菌培养箱，生化培养箱，恒温培养箱，真空干燥箱，鼓风干燥箱，超净工作台，生物安全柜，磁力搅拌器，离心机，超声波细胞粉碎机，超纯水机，高压灭菌器，实验室各种烧杯，电泳仪电源，实验台，恒温循环器，低温循环高空泵，农残速测仪，紫外分析仪，移液枪，PCR仪，液相色谱，气象色谱仪，酸度计/PH计，分光光度计，超低温冰箱，恒流泵，旋片式真空泵，自动部分收集器，低温恒温槽；等系列产品。

A basic knowledge of microbiology, will be detected with a microscope observation of micro-organisms, medium Preparation, disinfection and sterilization, separation and purification of micro-organisms, bacteria counting method A chemical analysis of the theoretical knowledge and can use chemical analysis methods and equipment of the components of material analysis Master fermentation process principles, understanding of beer, liquor, fermentation production technology Master protein related knowledge, PAGE gel electrophoresis technology, high-speed centrifugal Genetic engineering master the basic theory of knowledge, the extraction and purification of genes, PCR principle, gene recombination technology Master of the basic theoretical knowledge, the immobilized enzyme, the enzyme preparation Application Master of animal and plant tissue culture techniques of experimental steps studied experimental apparatus: Balance (scales, electronic scales, Analytical Balance), or acidity, spectrophotometer, liquid gas chromatography, acid-base titration apparatus, microscopes, centrifuges, ultrasonic cleaning, constant temperature oscillation incubator, super-clean table, steam sterilization pot, the pot constant temperature water bath, PAGE device apparatus, oven, fermenter, such as PCR instrument.

具备微生物理论基础知识，会用显微镜检测观察微生物，培养基的制备、消毒与灭菌，分离与纯化微生物，细菌的计数法具备分析化学的理论知识，能运用化学分析和仪器分析方法对物质的组分进行分析掌握发酵工艺原理，了解啤酒、白酒、发酵生产工艺掌握蛋白质相关知识，PAGE凝胶电泳技术，高速离心掌握基因工程的基本理论知识，基因的提取和纯化，PCR技术原理，基因重组技术掌握酶的基本理论知识，酶的固定化方法，酶制剂的应用掌握动植物组织培养技术的实验步骤学过的实验仪器：天平（台天平，电子天平，分析天平）、酸度计、分光光度计、液气相色谱仪，酸碱滴定仪器，显微镜，离心机，超声波清洗器、恒温振荡培养箱、超净工作台，蒸气灭菌锅、恒温水浴锅，PAGE电泳装置仪器、干燥箱、发酵罐、PCR扩增仪等。

Methods: Optics and electron microscope were used to observe the microstructure and ultrastructure of human apocrine sweat glands in axillae from 20 osmidrosis patients, and then discuss the relations between different gender, age, seasons arid odor intensity.

用光学显微镜、透射电子显微镜观察20例腋臭患者腋窝顶泌汗腺显微、超微结构，并分析性别、年龄、季节等发病因素与气味强度之间的关系。

The effects of high temperature tempering on the microstructure and mechanical property of an ultra-low carbon bainitic steel have been investigated by means of transmission electron microscopy, scanning electron microscopy, micro-hardness measurements and dilatometry.

本研究以穿透式电子显微镜、扫描式电子显微镜、微硬度量测及热膨胀仪分析超低碳变韧铁合金钢高温回火之显微结构及机械性质变化的特性。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

The mice eyes were enueleated and plastic-embedded and the anterior chamber angle were sagittally sectioned in 3 μm thickness. The morphology and structure of the eye tissues was studied by light microscopy and transmission electron microscopy.

通过塑料包埋切片技术，用光学显微镜和透射电子显微镜观察两组小鼠眼前房角组织的形态和超微结构。

The ultrastructure of spermatozoa of Takifugu xanthopterus was observed by means of scanning electron microscopy and transmission electron microscopy.

在透射电子显微镜及环境扫描电子显微镜下观察黄鳍东方鲀精子的超微结构。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。