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The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示,祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2,4-二硝基氯苯所致的小鼠迟发性超敏反应;大剂量时还可增加小鼠的胸腺指数及脾指数;止痒试验显示,祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间,并能增加豚鼠耐受磷酸组胺的致痒阈。

The total flavone in antioxidant substance extracted from leaves of tartary buckwheat and its scaveninging function to OH and O(superscript - subscript 2) were determined by photometry and the inhibitory action to Lipin peroxidation and its synergy with other substances were studied by iodimetry in the experiment.

从苦荞叶中提取抗氧化性物质,用光度法测定提取物中总黄酮的含量及对羟自由基和超氧阴离子O(上标-下标 2的清除作用,并采用碘量法研究了苦荞叶提取物对脂质过氧化的抑制作用及与其他物质的协同作用。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法:(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC,细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构;(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定;(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC,并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况,根据各个细胞克隆受Dox调控表达的程度,选择低背景、高诱导表达AT2R的细胞系,作为双重稳定MSC细胞系;蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况;(4)建立大鼠颈动脉球囊损伤动物模型,将双重稳定MSC在术中导入血管,分别于14 d、28 d进行病理切片,检测可调控AT2R对新生内膜增生的影响;采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变,TUNEL法检测血管组织中细胞凋亡的变化情况。

Using volatile acid and alkali to regulate the pH of pressed juice from bamboo shell,saltless Fraction Ⅰand Fraction Ⅱwere got separately.the residue of squeezed banboo shell was used as paper making material and medium for cultivation mushroom.

采用挥发性酸和碱调节鲜笋壳压榨液 pH值,分别获得无盐Fraction Ⅰ和fraction Ⅱ;其压榨后的蒲渣作为造纸原料和栽培食用菌的基质;对分离Fraction Ⅰ和Fraction Ⅱ后的汁液,加入Fe2 +并超滤浓缩,经硫酸铵盐析和丙酮分级后,进行CM 2 2纤维素离子交换柱层析,以 pH5 。2乙酸钠分级洗脱,获得RZ为 3 。0以上的高纯竹笋过氧氧化物酶。

The above facts indicated that silicon accumulation in the rice leaf sheath inside, the leaf surface and the sclerenchymatous cell, played the physical barrier role, delayed the hypha of sheath blight expansion.5、Analysing the physiological and biochemical mechanism of the resistance to rice sheath blight improved by silicon application in several respects.(1) After being inoculated with R.solani, O2 and POD activities disordered, CAT activities obviously declined. O2 producingvelocity in 91SP\'s leaves and leaf sheaths of Si+ rice plants were lower than those of Siriceplants, MDA content in leaves and leaf sheaths of Si+ rice plants were lower thanthose of Si- rice plants significantly in three days after inoculating, SOD、POD、CATactivities in leaf sheaths of Si+ rice plants were higher than those of Si- rice plantssignificantly in one day after inoculating, SOD、POD、CAT activities in leaves ofSi-treatment were higher than those of Si- rice plants significantly in three days afterinoculating. O2 producing velocity and MDA content in Lemont\'s leaves and leafsheaths of Si+ rice plants were lower than those of Si- rice plants significantly afterinoculating, and SOD、POD、CAT activities of Si+ rice plants were higher than those ofSi- rice plants significantly.

说明硅在水稻叶鞘内侧、叶表和厚壁细胞积累,起到了物理屏障的作用,延缓了纹枯病菌的扩展。5、阐明了施硅增强水稻抗纹枯病的生理生化机制(1)接种纹枯病菌后,两个水稻品种91SP和Lemont的叶鞘和叶片超氧阴离子自由基O2性紊乱,CAT活性明显下降;抗病品种91SP叶鞘和叶片施硅处理的O2产生速率小于缺硅处理,叶鞘和叶片MDA含量在接种第3 d后显著低于缺硅处理,叶鞘SOD、POD、CAT活性在接种1 d后一直显著高于缺硅处理,叶片SOD、POD、CAT活性在接种3 d后显著高于缺硅处理;感病品种Lemont叶鞘和叶片施硅处理的O2产生速率、MDA含量始终显著小于缺硅处理,SOD、POD、CAT活性始终显著大于缺硅处理。

Vitamin is most important function is as an antioxidant . Along with vitamin A , vitamin helps the body produce superoxide dismutase SOD , which scavenges for free radicals .

维生素E,一类由生育酚组成的脂溶性维生素,是非常重要的抗氧化剂,它能够与维生素A协同作用帮助人体产生超氧化物歧化酶,后者能够有效的清除体内的自由基。

Vitamin E is most important function is as an antioxidant . Along with vitamin A , vitamin E helps the body produce superoxide dismutase SOD , which scavenges for free radicals .

维生素E,一类由生育酚组成的脂溶性维生素,是非常重要的抗氧化剂,它能够与维生素A协同作用帮助人体产生超氧化物歧化酶,后者能够有效的清除体内的自由基。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。