起始的

Then we transfected transitorily the recombinant of green fluorescent protein gene and middle molecular weight neurofilament cDNA into wide type N2a (N2a/wt) and N2a/tau40 to observe the effect of tau accumulation on GFP-NFM fusion protein transport in cellular processes in living cells. At last we used an apoptotic inducer, camptothecin (an inhibitor of topoisomerase-1) to treat N2a/wt and N2a/tau40 cell lines, and compared their apoptotic response.

主要结果如下：一、tau蛋白过度表达和聚积对细胞形态的影响：倒置显微镜下观察两种细胞的形态，发现N2a/wt细胞的突起多而长，而N2a/tau40细胞胞体变圆，突起明显缩短；免疫印迹结果显示转染了tau40的细胞内tau的免疫反应约增加14倍，免疫荧光结果显示N2a/tau40细胞胞体内呈现出较强的红色荧光，tau主要分布在核周和突起起始部分的胞质内，而N2a/wt细胞内的荧光很弱。

The result of whole mount in situ hybridization showed that MmeFer was located at the position of shell initiation in trochophore stage, indicating MmeFer plays a role in shell initiation in M. meretrix. The full-length of M. meretrix cathepsin B cDNA was cloned with 3'and 5'RACE. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. From L2 to L4 stages, MmeCB mRNA was detected in the digestive tract, suggesting a possible role of MmeCB in digestion.

根据构建的文蛤幼虫cDNA文库中提供的序列信息，从文蛤中克隆了与铁离子代谢密切相关的铁蛋白的全长cDNA序列；通过Real time PCR发现，MmeFer mRNA的表达量在贝壳形成前后有明显改变；整体原位杂交结果显示MmeFer mRNA在L1期的表达部位刚好是贝壳生成的起始部位，推断文蛤铁蛋白与文蛤幼虫贝壳初始形成密切相关。

The first is to choose any one of the impossible shortest path but it doesn't eliminate any of the impossible shortest path by passed top clusters to unpassed top cluster.The second is tha...

而Dijkstra原始算法仅在可能存在的多条最短路径中任选其中一条即可；2、Dijkstra算法的每一步骤，不仅要求路径最短，同时还要求经过的顶点最少，从而求出被原始算法忽略的所有可能存在的最短路径；结果最终可以求出带权图中一起始点到其余顶点的所有最段路径。

Referring to a location in one of your own sections using wrt ..gotoff will give the distance from the beginning of the GOT to the specified location, so that adding on the address of the GOT would give the real address of the location you wanted.

用wrt 。。gotoff来引用你的某一个段中的一个地址，实际上得到的是，从GOT的起始地址到你指定的地址之间的距离，所以这个值再加上GOT的地址才是你要引用的实际地址。

When a strong and slowly degrading scaffold is used, the starting position of alular tissue engineering is a stronger ale and seeded cells are allowed more time to create a strong extracellular matrix.

当使用一个强壮的降解缓慢的支架时，瓣膜组织工程的起始点是一个更加强壮的瓣膜，种植的细胞有更多的时间创造强壮的细胞外基质。

As it can be expressed easily by argument equations, we use the conicoid as the criteria of points" classification. Based on the point"s normal and the adjacent field, corresponding to the conicoid equation such as plane, ball and cylinder, we search the points that fit the equation along the adjacent field. Using this method, we realized the automatic classification of unorganized points.The experiments using the algoritlim and criteria approved from this thesis are also carried out.

本文采用了可以比较方便地用参数方程表示的二次曲面作为数据点的分类标准，根据点的法向量以及其邻域结点的各种属性，对应于平面、球面、柱面等二次曲面，依据曲面方程的特点，从起始点开始沿邻域深度优先递归寻找符合方程的数据点，实现了散乱数据点的自动分类。

Results The tristar of groove was consisted of beak of the ethmoidal bulla and its prolonging process, apex of the uncinate process and anterior peak of the middle turbinate, at the region of frontal recess. The structures called meatal groove, uncinate groove and bullar groove were observed around the ethmoidal bulla and the tristar of groove located at their origination. Under endoscopic view these structures looked like a triangular clefts, so it was named.

结果 星状裂是由筛泡的顶端及其向上伸出的角、钩突的顶端、中鼻甲的前顶端在额隐窝区域汇合形成的一个星状裂隙，上述结构围绕筛泡形成鼻道沟、钩突沟、筛泡沟，星状裂为三条沟的起始部。

According to the structure-activity relationships of actinonin and the theory that drug binds with acceptor, we designed and synthesized three series of novel hydroxamic acid derivatives in which the isoxazole ring were contained. Their structures have been confirmed by 1HNMR, MS and other analytic methods.

本文就是以天然放线菌素为先导化合物，根据放线菌素的构效关系和与PDF受体结合的原理，以易得的氨基酸为起始化合物，采用了不同的合成路线设计合成了三类新的含有异噁唑杂环的异羟肟酸衍生物，并通过1HNMR，MS及其他分析方法验证了它们的结构。

It is not ignorable that there is base datum with less precision to support the network computation and adjustment of a constrained GPS network.

在处理如GPS控制网的约束平差或以GPS测定导线网的方位角的平差计算中，是否考虑起始数据的误差给平差结果及其精度评定带来的影响是不可忽视的。

There is evidence indicating the existence of an innate migratory drive as well as genetic control of the onset, duration and end of the migration period, the amount of migratory activity, a genetically prescribed parameter that determines the distance over which the bird flies, the migration directions and physiological parameters, in particular fat deposition during the migratory period, but also those determining which individuals will migrate and which will not in forms that are obligatorily partial migrants.

有证据表明，存在某种先天的迁徙动因并涉及以下几方面的遗传调控：(1)迁徙过程的起始、持续以及结束；(2 )迁徙活动量，即决定鸟类飞行距离的遗传参数；(3)迁徙方向；(4)生理参数，特别是迁徙期间的脂肪贮存，以及对于那些部分个体迁徙的鸟种而言，决定个体迁徙与否的生理参数。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了