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Tebufenpyrad, a new high effective pyrazoleamide acaricide , was synthesized from 1-methyl-3-ethyl-4-chloro-5-pyrazolecarbon acid and 4-t-butylbenzylamine, the raw material are propionyl pyruvic ethyl ester and 4-t-butyl benzylchloride.

吡螨胺是一种新型、高效的吡唑酰胺类杀螨剂,本文以中间体1-甲基-3-乙基-4-氯-5-吡唑甲酸和4-叔丁基苄胺合成,起始原料丙酰丙酮酸乙酯和4-叔丁基苄氯,总收率为61.23%。

The initial concentrations of acenaphthene in soils were 35.0 mg/kg.

供试污染土壤中危的起始浓度为35.0 mg/kg。

In the presence of Ru, the onset of methanol adsorbate oxidation is shifted to the more negative potentials.

在Ru存在下,甲醇吸附物在Pt电极上氧化的起始电位向负电位移动。

If you are using Inhalation Aerosol , then the usual starting dose is 2 to 3 inhalations .

如果您是使用吸入气雾剂,然后惯常的起始剂量是2至3吸入。

Then Doripenem came into the market in the July ,2005. We studied the synthesis of Doripemen.Firstly, we synthesized the chemical 2 from the starting material------o-hydroxylphenylacylamine through Reformastky reaction, alkylation, Diekmann reaction, enolization, esterification and etc. Secondly, we synthesized the chemical 3 from L — hydroxylproline through the protection of carboxyl, amidogen and hydroxyl group, reduction by NaBr, SN_2 substitution and Mitsumobu reaction.

我们以水杨酰胺为起始原料,经Reformatsky反应、烷基化、Diekmann环合、烯醇化、酯化等反应合成双环母核2;再从L-羟基脯氨酸出发,经酯化保护羧基、保护氨基、保护羟基、硼氢化还原酯得醇、Sn2取代和Mitsumobu反应等合成巯基侧链3;最后由化合物3经脱保护、水解得硫醇,和化合物2在二异丙基乙基胺的作用下缩合,最后Pd/C催化脱保护,历经16步反应最终得到产物多尼培南。

Your starting salary is 13,000 per annum and will be reviewed annually.

你的起始工资是每年13,000英镑,然后每年审核一次。

The results showed that both arsenate and arsenite compounds could be removed efficiently by NZVI at pH=7 and under 20℃.

结果表明,在pH为7,温度20℃时纳米铁能够快速地去除As和As,在60min内,0.25g纳米铁对起始浓度为968.6μg/L As和828.9μg/L As的去除率大于99.5%。

N-succinimidyl 5---3-pyridinecarboxylate, a useful linker for labeling proteins with astatine and iodine radioisotopes, is synthesized in three steps with 5- bromonicotinic acid as a starting material, and is characterized by nuclear magnetic resonance, mass spectrometer and high performance liquid chromatography.

以5-溴烟酸为起始物,通过3步主要反应合成了一种适用于蛋白质放射性砹标记的偶联剂——5--3-吡啶甲酸 N-琥珀酰亚胺酯,并用核磁共振、质谱、高效液相色谱等进行了表征。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平,用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量,并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用;采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株,研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

The summary results are below:1. GUS expression under the driving of the BjCHI1 promoter (-1060/+17) was essentially undetectable in the young seedlings under normal growth conditions. GUS activity was first detected in the stigma of young flowers, peaked in the young siliques, and decreased when the siliques became older. No GUS expression was found in the mature siliques, seeds or root.2. The BjCHI1 promoter (-1060/+17) was inducible by NaCl, PEG, wounding and MeJA treatments. High levels of GUS expression were detected in the transgenic tobacco and Arabidopsis plants after wounding, NaCl, PEG, and MeJA treatment, indicating that the BjCHI1 promoter responses to both biotic and abiotic stresses.3. RT-PCR analysis confirmed that the expression of the BjCHI1 gene in B. juncea was inducible by PEG and NaCl.4. The transcription start site was determined by 5′-RACE, and was located at the 17th nucleotide upstream of the translation initiation codon of the BjCHI1 gene.5. A -805/+17 promoter fragment was enough to response to wounding and MeJA induction, which was proved in transgenic tobacco and Arabidopsis plants. The 397 bp region between -805 and -409 of the BjCHI1 promoter contains a cis-acting element that is essential for the wounding and MeJA inducibility.6. The -695/-620 region was necessary but not sufficient to confer MeJA-responsive expression. A T/G-box locates in -353 play an important role in the expression of the BjCHI1 gene in response to MeJA treatment. The 76 bp region is coupled with the T/G-box to confer full MeJA-inducible transcription of the BjCHI1 gene.

主要结果如下:1、利用转基因拟南芥植株分析表明,正常生长条件下,BjCHI1启动子(-1060/+17)驱动GUS基因主要在花柱中表达,幼嫩的荚也有表达,并随着果荚的成熟而减弱,成熟的果荚、种子和根没有显示GUS活性。2、BjCHI1启动子(-1060/+17)能驱动GUS基因在转基因烟草和拟南芥中响应伤害的诱导,转基因拟南芥的分析还证明BjCHI1启动子也受MeJA、NaCl和PEG的诱导,证明BjCHI1启动子是一个伤害、MeJA、NaCl和PEG等生物和非生物因素诱导启动子。3、RT-PCR进一步证明芥菜中BjCHI1基因也受NaCl和PEG的诱导表达。4、5′-RACE法鉴定了BjCHI1启动子的转录起始位点,位于翻译起始位点ATG上游第17个碱基A.5、转基因烟草和拟南芥分析证明,-805/+17的启动子片段足以响应伤害和MeJA的诱导,-805和-409之间397 bp的启动子片段含有对伤害和MeJA诱导必要的元件。6、本明烟叶片瞬时表达系统分析证明,一段76 bp的序列(-695/-620)对BjCHI1启动子响应MeJA的诱导是必要的,但不足以响应MeJA的诱导,位于-353的T/G-box也参与MeJA的诱导。76 bp的序列(-695/-620)与T/G-box协同起作用,赋予BjCHI1启动子MeJA诱导性。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。