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To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子,以色谱法分离纯化后进行体外活性研究,构建表达载体pPICZαA- ALR,经电穿孔转入毕赤酵母中,用0.5%甲醇诱导表达;重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明, rhALR以分子量为30kD的二聚体为主;定量分析结果表明,重组酵母培养上清中rhALR约占总蛋白的66%,表达量约为40mg/L;经DEAE柱和G75柱纯化后,获得的rhALR纯度大于95%,得率为52%;体外生物学活性实验表明,rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Based on low satellites on orbit and STK platform, some parameters of space-based optical system such as observation direction, Right Ascension of the Ascending Node and True Anomaly are set and optimized in this paper, which will lay a foundation theoretically for the design of space-based optical system.

本文以在轨运行的低轨卫星为研究对象,以卫星工具包STK为平台对天基光学系统的观察方向、升交点赤经和真近点角等参数进行了优化设置,为天基光学系统的设计任务奠定理论基础。

Astronomy the angular distance of a celestial point measured westward along the celestial equator from the zenith crossing; the right ascension for an observer at a particular location and time of day.

从天顶交叉口向西顺着天赤道测量的一个角距离;对在一个特定的地点和特定的时间观测者来说是赤经。机会难得!足额稳定绿色激光笔团购!

This paper firstly discusses the constellation rotation error of autonomous orbit determination of navigation satellites which only make use of crosslink ranging observation, then analyzes in depth the influence of tide perturbation on right ascension of the ascending node and autonomous orbit determination.

讨论了仅利用星间测距观测值进行导航卫星自主定轨时所遇到的星座整体旋转问题,分析了潮汐摄动对升交点赤经以及导航卫星自主定轨的影响。

SRA,/* Sun's Right Ascension

同前,太阳赤经

Re-tighten the graduation-ring setscrew.

重新上紧赤经兼小时刻度环的定位螺丝。

Namety, under the action of J2 perturbation, relative right ascending node, argument of perigee and mean anomaly of satellites formation will drift secularly.

首先,分析J2摄动对编队卫星相对轨道要素的影响,即J2摄动使相对升交点赤经、近地点辐角和平近点角产生长期漂移。

The determination method of descending/ascending node, longitude of ascending node and frozen orbit parameters for sun-synchronous orbit satellite design was analyzed on the basis of six general orbital elements.

在轨道六要素的基础上,分析了卫星太阳同步轨道设计时降交点地方时、升交点赤经,以及冻结轨道参数等的确定方法。

The configuration stability of the elliptical orbit satellite constellation requires the stabilization of the ascending node right ascension, the argument of perigee and the mean anomaly at the same time.

椭圆轨道星座构型稳定性要求同时实现升交点赤经、近地点幅角和平近点角的稳定。

Because of the secular perturbation variations of the ascending node right ascension, the argument of perigee and the mean angle caused by the initial deviations of the semi-major axis, the inclination and the eccentricity are linear time-variant, then the active biases of the semi-major axis, the inclination and the eccentricity can realized the self-stabilization design of the elliptical orbit satellite constellation, and so the configuration stability will be improved.

通过初始偏差对椭圆轨道卫星的长期影响分析可知,轨道半长轴、倾角和偏心率的初始偏差对升交点赤经、近地点幅角和平近点角的长期摄动变化是线性的,因此通过主动偏置轨道半长轴、偏心率和倾角能够实现椭圆轨道星座构型的自稳定设计,从而提高其构型稳定性。

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