贴用

ABSTRACT:The therapeutic group was compared with 5 control groups, which were normal group, ventolin treatment group, ion-guide group, external application group and model group.

用复方麻辛酊敷贴离子导入疗法对哮喘豚鼠进行治疗，并与正常组、喘乐宁对照组、离子导入组、敷贴组、模型对照组相比较，观察各组动物白蛋白激发后气道阻力和肺顺应性的变化。

The simple algebraic procedure is used to form nearly orthogonal 2—D C—type and H—type bodyfitted grids for bodies of revolution, which is then used to generate 3—D C—O and H—O bodyfitted grids for fuselages.

五、用简单的代数方法生成了近于正交的旋成体子午面"C"型及"H"型贴体坐标网格，并以此为基础构造了三维任意截面机身的"C-O"型及"H-O"型贴体坐标网格。

When the color map is done I will take a copy of that and use itfor the base of the specular map.

当颜色贴图做好后我做一个备份，用它去做张基础的高光贴图。

Textures are assigned in the diffuse map channel of the Blizzard material.

贴图用暴雪的专署材质赋予色彩贴图通道。

To save cloth, mom collects a shell of bamboo shoot of the fallen that be blown by wind in oneself Zhu Lin, what go out with millet straw rub is downy, roast hind planish, with 2, carapace of three-layer bamboo shoot is stuck case, use needle and line to plunge into, cut shoe pattern next, do the surface layer of upper with the La Bu that already caught oneself, what do sole with the plain white cloth that he weaves is outer, and the middle-level of upper, sole is to use wear the old cloth that rotted the old clothes that cannot fill again is torn open, paste has an one layer land, after been cut according to shoe, seam good Bian Zi with needlework, this sole make it.

为了节省布料，妈妈就在自家竹林里捡回被风吹落下来的笋壳，用谷草搓去外面的绒毛，烘烤后压平，用二、三层笋壳贴起，用针和线扎起，然后剪成鞋样，用自已染的蓝布做鞋帮的表层，用自己织的白布做鞋底的外层，而鞋帮、鞋底的中层都是用穿烂了不能再补的旧衣服拆的旧布，一层一层地用浆糊粘起，照鞋子剪好后，用针线缝好边子，这鞋底便做成了。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

When I'm satisfied with the general volume a shell modifier helps me to give thickness to the piece. In this phase, I don't usually bother that much about every single rivet, hole or cut, I just try to develop a good quad topology of the main volumes. Of course the topology is done by taking count of the main cuts and holes, but the rest can be done easily with a normal or bump map. It's up to you to decide how much a single detail is worth to be modeled or can it be put into a bump map. I usually adopt a "comfortable" criteria...it means that if something looks tricky to me to be carved or extruded into an existing geometry I usually put it into a normal map. When you make something for production, you don't usually have much time to model everything, so you need to set priorities.

当我对整个盔甲的体积感满意之后，一个shell修改器就可以帮我给整个盔甲添加厚度，在这个阶段，我通常不会考虑太多那些细小的铆钉，洞眼或者切边，我只是给整个的模型确立一个好的四边形拓扑结构，当然拓扑是通过重视主要的切割和打洞来形成的，但其余那些细小的部分可以用一个normal 或者bump贴图很容易地模拟出来，这由你来决定到底一个细节是值得建模还是放到一个bump贴图里面，我通常采用一个&舒服&的标准，也就是说，如果有什么东西对我来说，在已有模型上再挤出或者开洞是非常麻烦的，那么我就通过Normal贴图来表现，而当你为商业生产制作模型时，你通常都没有足够的时间来把任何东西都建出来，因此你需要设置重点。

Peel off the Onuge Strips from the hard plastic film and then paste to the teeth gently each time for10 - 20 minutes, two times each day , not affecting talking, not interfering with the work and may be used in any time. Use clean water for gargling after sevice.

在使用欧诺洁深层美白牙贴时，只需将牙贴从硬塑膜上剥离，轻轻的贴到牙齿上，每次15-30分钟，每天两次，不影响说话，不影响工作，适合日常的使用，使用后用清水漱口即可。

Peel off the Onuge Strips from the hard plastic film and then paste to the teeth gently each time for10 - 20 minutes, two times each day , not affecting talking, not interfering with the work and may be used in any time. Use clean water for gargling after sevice.

在使用欧诺洁深层美白牙贴时，只需将牙贴从硬塑膜上剥离，轻轻的贴到牙齿上，每次15—30分钟，每天两次，不影响说话，不影响工作，可以在任何地方使用，使用后用清水漱口即可。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母细胞时，将同周期成熟卵丘复合体切出部分卵丘细胞，用1 mL注射器抽打分散细胞，贴壁培养。113个治疗周期中，298枚生发泡期卵母细胞经3种不同培养液体外成熟培养(同一病人的生发泡期卵被随机分到某同一组中)：第1组28个周期中73枚：基础培养液+卵泡液；第2组40个周期中115枚：A液+分散贴壁的卵丘细胞；第3组45个周期中110枚：A液+分散贴壁的卵丘细胞+促卵泡生成激素+表皮生长因子。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。