质粒体

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Ultrastructurally, the chalazal border of the mature egg cell demonstrates regularly discontinuous regions in which exist a great number of single or double-membrane vesicles.

卵细胞中的质体多含淀粉粒，精细胞中的质体均不含淀粉粒。DNA荧光显示，卵细胞质中具丰富的类核并可以断定环状类核为线粒体DNA。

Electron microscope observations showed that the liposome suspension was heterogeneous and the particle size ranged from 20 nm to 700 nm with a average diameter of 163 nm. The vesicles showed unilamellar and multilamellar structure which consisted of several concentric bilayers.

电镜观察表明，该脂质体多呈球形的单层或多层结构，脂质体粒径分布均匀，粒径范围20-700 nm，平均粒径163 nm，主要粒径分布位于50-200 nm(84.80%)。

However, relating to investigate the modes, courses and characteristics of the orogenetic process in Paleozoic and after orogenetic process, the cognitive divergence have still occurred.

通过对新疆北东部地区不同构造区带构造作用的方式、过程和特点的研究，确定：①南天山榆树沟高压麻粒岩地体的正变质原岩是一个基本完整的蛇绿岩套，其经历了俯冲至下地壳的高压麻粒岩相、构造抬升至深地壳麻粒岩相以及快速构造折返至角闪岩相的变质过程，代表了南天山洋壳俯冲、变质、随后折返的过程，并首次确定了变质过程的PTt轨迹；②奥陶纪末期中天山北缘发生洋盆俯冲、地体增生作用，此时萨日达拉地区处于岛弧活动带；中石炭世晚期中天山小型洋盆关闭，准噶尔地块与伊犁—中天山板块斜向对接随后发生脆韧性质近水平高温低速右行走滑剪切；③根据巴音沟地区不构成蛇绿岩套有效成分的硅质岩具有与活动大陆边缘密切相关的地球化学特征以及洋中脊玄武岩显示其岩浆源区受到消减带流体交代作用的影响，结合区域地质资料，首次提出巴音沟蛇绿岩带所代表的洋盆古构造环境为弧后盆地，并于早石炭世至中石炭世末期间，经历了扩张、收缩和闭合过程；④首次在克拉麦里蛇绿混杂岩带中分解出具有N-MORB、T-MORB与OIB三种不同类型的火山岩岩石组合，反映了洋盆俯冲

The reservoir rock types in the region of interest are grain limestone, algal limestone, cryptite, siltstone, muddy limestone and mudstone. The thesis identified as a result of changes in the deposition causing the heterogeneity in plane, and then the geological oil reserves have been calculated in accordance with the areal heterogeneity.3、Based on the data of core analysis, bore logging, array induction imaging log and FMI, porosity and permeability model were built using regression analysis. Through testing 45 rock samples, a, b, m, n value were obtained and used to calculate single-layer oil saturation value using the Archie formula. According to the theory of laterolog and inductolog, resistivity ratio and cross plot were used to identification effective thickness. Effective thickness was identified delicately in 103 wells. Based on the over work, active porosity and oil saturation was calculated accurately.4、According to observation of 100 pieces of the thin slices, cast thin slices and 383m core in three wells, the chief diagenesis in this area were compaction, cementation, denudation and cataclasis. Cementation and denudation were universally influenced active porosity this area. The intergranular pores, residual pores and secondary pores were formed in thin carbonated reservoir of lacustrine facies.5、The influence of reservoir heterogeneity to reservoir calculation was analyzed systematically using actual data. And the conclusions were as follows:①The principal reason leading to oil distribution unequal is interior structural feature of reservoir in structural hydrocarbon reservoir.

确定了由于沉积微相的变化而导致的平面非均质性，进而在平面上按照平面非均质单元计算了石油地质储量。3、利用岩心分析、常规测井曲线与阵列感应、成像测井资料相结合，分岩性利用回归分析方法建立了储层孔隙度、渗透率模型；通过对45块岩样进行岩电试验，获取了a、b、m、n值，利用阿尔奇公式计算了各单层含油饱和度值；根据侧向电阻率和感应电阻率串并联特性，利用电阻率比值法与常规交会图法相结合重建了有效厚度图版，对全区103口井目的层段进行了有效厚度精细划分，通过以上工作，准确求取了储量计算单元内有效厚度参数、有效孔隙度参数以及含油饱和度参数。4、通过对研究区3口井383m岩心观察，100余块薄片、铸体薄片镜下鉴定得出：油泉子油田上、下油砂山组储层主要成岩作用是压实作用、胶结作用、溶蚀作用和破裂作用，其中溶蚀作用、胶结作用最普遍，这两种作用对有效孔隙度影响最大，以上成岩作用形成了原生粒间孔、粒间残余孔和次生孔隙(如粒间溶孔、粒内溶孔、铸模孔、晶间孔等)为主的薄层湖相碳酸盐岩储层。5、利用实际资料，系统分析了油藏非均质对储量计算的影响，认为：①对于构造油气藏而言，油气藏构造及断裂特征是造成油气分布不均的首要原因，储集条件相同的条件下，构造高部位有利于储存油气，油气在垂向的重力分异体现了油气藏内部构造的不均一特征。

The granulite xenoliths, as the product of basaltic underplating and the representative of the present-day lower crust, are different from the local Archean granulite terrains There is a systematic variation of Sr-Nd isotopes from the granulites to the pyroxenites and the peridotites, indicating the possible crust-mantle interaction beneath Hannuoba area.

麻粒岩类捕掳体所代表的年轻大陆下地壳为玄武质底垫作用的产物，与麻粒岩地体所代表的古老大陆下地壳不同。橄榄岩类捕掳体则具亏损特征，辉石岩类捕掳体的性质介于麻粒岩类和橄榄岩类捕掳体之间，但石榴子石辉石岩显示了其特殊性。

According to their mineral assemblages, the granulite xenoliths entrained by Cenozoic Hannuoba basalts are divided into four groups: felsic granulites, noritic granulites, two-pyroxene two-feldspar granulites and two-pyroxene granulites, with the two-pyroxene granulites most common.

根据矿物组合特征，汉诺坝玄武岩中的麻粒岩捕掳体可划分为四种类型：长英质麻粒岩、苏长质麻粒岩、二辉二长麻粒岩和二辉麻粒岩，其中以二辉麻粒岩最为常见。

FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3 mediated by PEG/LiAc and by electroporation . In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .

把FDR3基因片段克隆到酵母表达载体pDBLeu上，然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体（M3 mutant）中，采用酵母异源互补法进一步鉴定FDR3基因的功能。

Researchers in Japan showed that rings of DNA called plasmids could also carry the required genes into the cell.

日本的研究人员揭示名为质粒的DNA环状体也可以将所需基因载入细胞。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端，克隆入pcDNA3.1构成DN突变体真核表达质粒。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！