质粒

In this paper the conception of horizontal gene transfer was introduced,and main mode of HGT was also enumerated as follows:HGT by medium such as plasmid and virus etc.and the HGT without any medium.

简要介绍了水平基因转移（horizontal gene transfer，HGT）的基本概念以及进行转移的主要方式：由质粒或病毒等介导的水平基因转移和基因的&直接&水平转移。

Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.

大量提取纯化重组真核表达质粒pVAX1-GRA8，瞬时转染培养的vero E-6细胞，RT-PCR和免疫印迹法检测GRA8在细胞中的表达。

The African green monkey kidney cell line, Vero, was used as the host for plasmid DNA and MNNG (N-methyl-N′-nitro-N-nitrosoguanidine) was chosen as the DNA-damaging agent.

实验采用非洲绿猴肾细胞株Vero和MNNG(N-methyl-N′-nitro-N-nitrosoguanidine)分别作为质粒DNA的宿主和损伤剂。

Methods: Oocytes in the GV stage were separated from ovary by squeezing method. In mouse germinal vesicle GV stage, the expression of ATP8 gene in the mitochondria in the single oocyte was detected by RT-PCR, in which, cDNA was synthesized with two methods: one was the single GV-stage oocyte directly to be placed RT, the other was to perform RT after eliminating mtDNA and nucleus DNA with the EeoR Ⅰ enzyme and Dnase. And the product of RT-PCR was cloned and sequenced.

应用挤压法从卵巢中分离获得生发泡期(germinal vesicle， GV)卵母细胞；用RT-PCR检测GV期单个卵母细胞中ATP8基因的表达：其中cDNA的合成分两种方法进行：一是将GV期单个卵母细胞直接进行RT合成cDNA，二是先用DNA酶加EcoR Ⅰ酶祛除mtDNA和核DNA后再进行RT；回收产物构建克隆质粒并测序。

Abstract—A yeast plasmid was constructed to contain a hybrid GAL–CYC promoter, the NPTII neomycin phosphotransferase gene, and the FRT sequence between them.

摘要，酵母质粒构建了含有混合放射公益少年团启动，新霉素的NPTII 磷酸转移酶基因，并首次登记税它们之间的序列。

Deoxyribonucleic acid is an important biomacromolecule. It is a carrier of genetic information and a critical target for radiobiological effects.

选用HI 13串列加速器产生的不同传能线密度的7Li和12 C重离子，以不同的剂量对纯化的质粒DNA水溶液进行了辐照。

A eukaryotic expression plasmid of Fas／APO－1 gene was constructed successfully.

本实验成功地构建了Fas／APO－1基因的真核表达质粒。

Xanthus. The plasmid vector pZCY11 made it more convenient for the study on functions and the expressions of target gene in M. xanthus.

构建的质粒载体pZCY11不仅能够对目的基因进行功能的分析，而且能够同时通过报告基因分析基因的表达情况，可简化粘球菌中基因功能及表达情况的研究。

Xanthus DK1622 by PCR, and inserted the fragment into a site upstream of lacZ, resulting in the recombinant plasmid pZCY13. The plasmid pZCY13 was transformed by electroporation to DK1622, producing a mutant ZC16-18 ?

从黄色粘球菌DK1622基因组上PCR扩增MXAN1334基因内部部分片段，插入载体pZCY11上lacZ基因的上游，构建重组质粒pZCY13，将其转入DK622菌株，获得MXAN1334基因插入失活突变株ZC16-18。

89 Strains of shigella isolated from 3 different areas( Chengtu clty,Yuxi district,Da Xingang)in 1990 were analysed by plasmid profiles and antibiotic resistance patterns.

本文对成都市、玉溪地区、大新港三地1990年分离的89株痢疾杆菌进行了质粒图谱分析及耐药谱分析。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！