质粒

Genes located on chromosomes, plasmids, or transposons encoding specific resistance to a variety of metal ions.

对重金属产生抗性的基因位于微生物的染色体、质粒和转座子上。

By restriction enzyme digestion and gel extraction, any of five antibiotic resistance genes with specific ends can be conveniently obtained.

通过限制性酶切和胶回收技术，可以从上述构建的质粒中方便地获得带有理想接头的抗生素抗性基因。

We had to extract recombine vectors to electrophoresis, prepare Biotin-probe to Dot Hybridization, check Nucleotides sequence etal.

此杂交过程除了重组子的质粒提取和DNA斑点印迹的手工制备以外，其余杂交步骤与第一部分相同。

Objective To study the effect of human insulin gene modified fibroblasts on blood glucose in diabetic rats.

目的 研究人胰岛素基因表达质粒转染鼠成纤维细胞后对糖尿病大鼠血糖的影响。

METHODS: Fulllength or fractions of VP22 were fused to C terminal of HBV core protein, and cloned into pcDNA3.1 vector, yielding eukaryotic expression plasmids of DN mutant.

将VP22全长及其不同区段融合于HBV核心蛋白的C端，克隆入pcDNA3.1构成DN突变体真核表达质粒。

Objective To construct the eukaryotic expression vector for pathogenic gene MYOC of primary open angle glaucoma and express in COS-7 cells.

目的 构建原发性开角型青光眼致病基因MYOC的真核表达质粒，并在COS-7细胞中表达MYOC蛋白。

In order to express the recombinant peptide of both gp52 and pp150 Oterminal peptides from human cytomegalovirus in the Pichia pastoris, the recombinant plasmids were transformed into Pichia pastor is by electroporation after linearised respectively by Sac I or Bg1II, the transformants were screened in MD plates without histidine.

为了在巴斯德毕赤酵母中表达人巨细胞病毒gp52C末端和pp150C末端串联片段的嵌合肽，将构建好的hCMVp-pPIC9K重组质粒分别经Sac Ⅰ和Bg1 Ⅱ酶切，电打孔法导入毕赤酵母GS115后，在缺组氨酸的MD板上筛选出转化子。

Using plasmid pUT 649 as a model plasmid,the extraction of DNA by reversed micelles system of methyltrioctylammoniumchloride/2-ethylhexanol/isooctane was studied.

采用新的TOMAC/2-乙基己醇/异辛烷反胶团体系，对该反胶团萃取质粒pUT649进行了研究。

Precomputed comparisons can be selected from a database covering all the completed bacterial chromosome and plasmid sequences in the Genome Reviews database.

预计算的比对能够从覆盖所有完整的细菌染色体和质粒序列的一个数据库中挑选。

In this paper the conception of horizontal gene transfer was introduced, and main mode of HGT was also enumerated as follows: HGT by medium such as plasmid and virus etc. and the HGT without any medium.

摘 要：简要介绍了水平基因转移（horizontal gene transfer， HGT）的基本概念以及进行转移的主要方式：由质粒或病毒等介导的水平基因转移和基因的&直接&水平转移。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！