质粒

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind III. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536. The ligation mixture was transformed into EPI300 competent cells. A total of 5184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用Hind III部分酶解后回收40~60 kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

The genomic DNA of Streptomyces diastaticus SM33 was extracted and partially digested by Hind Ⅲ. The 40~60 kb high molecular weight DNA was extracted and ligated to pIndigoBAC536.The ligation mixture was transformed into EPI300 competent cells. A total of 5 184 colonies were obtained by white-blue screening.

提取淀粉酶链霉菌SM33基因组DNA，用HindIII部分酶解后回收40kb~60kb大小的高分子量DNA，与质粒载体pIndigoBAC536连接，电击转化EPI300感受态细胞，经蓝白斑筛选共挑取了5184个白色克隆。

These results suggested that AFP can be used as a target gene of DNA vaccine in heptocellular carcinoma therapy.

成功构建了能在真核细胞中表达的重组质粒phAFP，为AFP DNA疫苗治疗肝癌的研究打下基础。

Gene therapy ; DNA vaccine ; plasmid ; ion exchange chromatography

基因治疗； DNA疫苗；质粒；离子交换色谱

In recent years, it was used to investigate the mechanism of bacteriocin as an intermediate vector, to construct lactic acid bacteria genetic strains and develop oral vaccine, even in gene therapy study.

已用于研究细菌素作用机制，乳酸菌基因工程菌株的改造以及口服疫苗的开发等，应用领域十分广泛，已成为乳酸菌基因工程研究的重要工具质粒之一。

The recombinant plasmid pBI121-VP6 was transformed into Agrobacterium tumefaciens strainLBA4404 by freeze-thawing method and the introduced into the sterile tube culture shoot of tobacco NC89 and baoding alfalfa by leaf disc infection method.

以冻融法将pBI121-VP6重组质粒导入根癌衣杆菌LBA4404，用叶盘法转化于NC89烟草和保定紫花苜蓿的无菌试管苗。

Used the method of touch down PCR, the ACS2 gene was cloned from DNA of Petunia hybrida. At the same time, its gene sequence DQ090003 was obtained in GenBank, the antisense repetition of ACS2 linked the plasmid pBI-121 by use of one step. In the result, the expression vector having antisense repetition gene was constucted.

利用降落PCR的方法，从矮牵牛DNA中扩增出ACS2基因片段，在GenBank中获得基因序列号为DQ090003；利用一步法将ACS2基因的反义重复与pBI-121质粒连接，构建含该基因的反义重复表达载体。

Indirect ELISA was used to test the antibody titer of the serum.

用重组质粒对Balb／c小鼠进行免疫，采用间接ELISA法分析特异抗体的产生。

The recombinant plasmid was transformed into its host E.coli strain BLai and a protein band with MW of 27kD was expressed.

将重组质粒转化大肠杆菌BL_(21)株，经IPTG诱导，表达分子量约为27KD的融合蛋白。

And to identify by nucleotide sequencing and restriction endonuclease digestion.(2) The recombinant plasmid was transformed into Bacillus coli and to shake culture.

将pET28a-hnRNPA2/B1重组质粒转化入大肠杆菌BL21振荡培养，用IPTG诱导表达融合蛋白并且优化条件，以SDS-PAGE分析融合蛋白的存在及可溶性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！