质粒

We then patch these genetic elements in the plasmid and insert it back into the bacterium, he added.

我们能够修改在质粒中的这些基因单元然后把它重新插入细菌的染色体中，他补充说。

It indicated that this four methods can obtain pure plasmid DNA.

表明四种方法均能获取纯化的质粒DNA。

This review focuses on the present status of application of polymer-mediated plasmid DNA transit systems.

本文对近年来聚合物介导的质粒DNA复合转运系统在骨组织工程中的应用进展做一综述。

Conclusion. 1.This plasmid as standard, the certification of the method can be used for both mutation detection.

结论1、以此质粒为标准品，验证了该方法可以用于这两种突变的检测。

New phages can develop by acquiring restriction enzymes from plasmids.

通过从质粒获得的限制性酶，新的噬菌体能够生长。

It was demonstrated that the dependence or independence of recA gene is not related to the direction of replication of the plasmids or phages in the free state.

实验结果说明，依赖与否和它们在游离状态中复制方向无关，从而否定了recA基因的作用是把质粒或噬菌体的游离状态的单向复制发动机构改变为整合状态的双向复制发动机构这一假设。

The strain CNB-1 has a circular chromosome of 5373643 bp in length and it encodes 4803 open reading frames, 3 rRNA operons, 79 tRNA genes for all the 20 amino acids.

本研究在已有工作的基础上，对该菌株的全基因组进行测序，该菌基因组包括一个环状染色体和一个大质粒。

Furthermore, we screened Jurkat cell lines which stably expresses sense and antisense IL-2Rαgene via neomycin selection.

同时，利用构建的质粒具有Neo抗性，用含G418的甲基纤维素半固体培养基进行了稳定整合反义IL-2Rα基因细胞株的筛选工作。

The capacity of the primary library was 5.0×105 PFU/mL and the capacity of the amplified library was 8×109 PFU/mL. The cDNA library was immunoscreened by the rabbit serum against H.qinghaiensis larval tick, the positive pluques were subcloned, and their plasmids extracted and then transformed into Escherichia coli JM109. Eventually, 11 genes were obtained and designated HqL1,HqL2,HqL3,HqL4,HqL5,HqL6,HqL7,HqL8,HqL9,HqL10 and HqL11,respectively.

采用常规方法构建了青海血蜱幼蜱cDNA表达文库，初级文库的库容量为5.0×105PFU/mL，扩增后的库容量为8×109PFU/mL；用兔抗青海血蜱幼蜱阳性血清对该文库进行了免疫学筛选，对阳性噬菌体进行了亚克隆，提取质粒后转化宿主菌JM109，最终共得到幼蜱基因11个，分别命名为HqL1、HqL2、HqL3、HqL4、HqL5、HqL6、HqL7、HqL8、HqL9、HqL10、HqL11。

The recombinant plasmid pQEM1 could steadily exist in the X4064 strain of S. typhimurium,without influence on its invasion into host cells.X4060（pQEM1）strain isolated from infected mice still had the ability to re-express M1 protein.

重组pQEM1质粒能稳定地存在于减毒鼠伤寒杆菌X4064株中，不妨碍其入侵宿主细胞，从小鼠组织中分离到的X4064(pQEM1)依然具有表达M1蛋白的能力。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！