质粒

Construction of heavy chain transgenic mice and genome type analysis Linearized transgene plasmids were microinjected into pronuclei of zygotes from CBA×C57BL/6 mice, and transplamted into oviduct of pseudo-pregnant mice.

二、抗体重链转基因小鼠建立及基因型分析将线性化的质粒显微注射入CBA×C57BL/6小鼠的受精卵细胞的雄前核内，然后导入假孕雌鼠的输卵管。

In view of these facts, We tried to find something in the L-form induction, plasmid extraction and their function, and the cloning and expression of promotors from BCG in Bacillus subtilis. Our aim is to provide a basis for the expression of HBsAg gene in BCG and to add something to the molecuiar biology of mycobacteria.

正是有鉴于此，我们试图在分枝杆菌在液体培养基中的L型实验诱导、质粒的提取及其功能分析、卡介苗启动子在枯草杆菌的克隆和表达等方面有所发现，为利用卡介苗表达乙型肝炎表面抗原基因提供基础，也为我国分枝杆菌的分子生物学研究增加内容。

The PTZ plasmid vector was changed to plasmid vector pSP72, which contains both T7 and Sp6 promotors for transcription of antisense (T7) and sense (Sp6) RNA probe in vitro. The results revealed the label rate of cRNA probe is high, the hybrids between RNA-RNA are stable, so the background of hybridization is satisfactory.

本实验将质粒载体PTZ转换为带有T7和Sp6两个启动基因的pSP72载体，故可在体外转录cRNA时同时得到意义（Sp6为启动基因）和反意义（T7为启动基因）探针，实验结果表明，cRNA探针标记率高，杂合物稳定，故所获杂交反应背景较为满意。

The construction of expressed plasmid in prokaryote not only obtained abundant protein but done research further for its separation, purification and biological activity.

构建斑马鱼GnRH原核表达质粒不仅获得大量的GnRH蛋白，还可对该蛋白做进一步分离纯化以及生物活性方面的研究。

In this study, the nattokinase gene was cloned into a prokaryon expression vector pET28a(superscript +) to construct the recombinant plasmid pETNK.

本研究将编码信号肽、前导肽和成熟肽在内的纳豆激酶基因克隆到原核表达载体pET28a中，获得重组表达质粒pETNK。

According to the sequence of rabbit defensin NP-1 and porcine defensin pBD-1 gene published in GenBank,We selected respectively prokaryon or eukaryotic expression vector to construct recombinant expression plasmid hoping for getting expression protein in E.coli or COS-7 cells, All these laid down a foundation in research of these two antibacterial peptides.

本文主要根据GenBank已公布的兔防御素NP-1基因和猪防御素pBD-1基因序列，分别选用原核或真核高效表达载体构建重组表达质粒，以期在大肠杆菌或COS-7细胞中得到高效表达，为进一步研究这两个抗菌肽奠定基础。

The introduction technique has beenindirectly proved on the molecular basis by the successfultransfer of marker genes and plastids.

外源标记质粒和目的基因的导入已对这一技术进行了间接的分子验证。

The EcoR digested of random recombinant plastids showed that each had been inserted by cDNA sequences.

随机挑取酶切质粒的电泳分析表明，载体中均有插入片段。

The bait plasmids constructed can be used to study the function of neuritin in Yeast Two-Hybrid Screen.

构建的诱饵重组质粒可以用于下一阶段的人胎脑cDNA文库筛选。

Plasmids that cannot exist in the same host cell belong to the same incompatibility group.

不能在相同的宿主细胞中共存的质粒属于同一个不相容组。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！