质粒

Transfect BHK-21 cells with screened positive recombinants and determine the expression of GFP and transcription level of 2B gene.

将阳性重组质粒转染BHK-21细胞，检测绿色荧光蛋白的表达和2B基因转录水平。

In this study, shuffling test and tetrads analysis were carried out to examine the complementation between Lag1p and LASS2 or its fragment containing TLC domain but excluding HOX domain (LASS2 △ HOX).

本实验通过质粒丢失实验和四分子分析实验分别检测了全长LASS2，去除HOX domain而保留TLC domain的LASS2片断(LASS2ΔHOX)与Lag1p功能互补的可能。

You have isolated a plasmid DNA that is a closed circular molecular 1050 bp in length with 5 negative supercoils.

你分离出的质粒 DNA 为1050 bp 长度的共价闭合环状分子，具5个负超螺旋。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果，选取LTR 变异率较高的U3 区，以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换，构建EIAV 非编码区强弱毒嵌合的全基因分子克隆，将阳性重组质粒命名为pLGFD9-12。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

Objective To acquire enough envelope glycoproteins so as to facilitate a further study of the structure and function of envelope glycoproteins from various kinds of virus isolates. Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

目的 获得足够量的膜糖蛋白，以便于对不同HIV分离株膜糖蛋白的结构与功能进行进步的研究方法从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人免疫缺陷病毒1(HIV-1)HXB2分离株原病毒基因组的重组质粒 pHXB2中克隆了两段膜糖蛋白基因片段。

Methods Two envelope glycoprotein gene fragments were cloned from the recombinant plasmid pHXB2 containing the human immunodeficiency virus 1(HIV-1) proviral genome of HXB2 isolate.

从人溃疡免疫缺陷病毒发烧1(HIV-1)HXB2分离株原病毒发烧基因组的重组质粒pHXB2中克隆了两段膜糖蛋白基因片段。

This recombinant plasmid will be a useful tool in the studies of the inductive mechanism of λ prophage and the repair of cells damaged by UV-irradiation.

这种重组质粒将在进一步研究λ原噬菌体的诱导机理以及受紫外辐射损伤细胞的修复作用等方面成为有效的工具。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！