质粒

Objective To construct recombinant plasmid of Legionella pneumophila mip gene and detect its expression in NIH3T3 cells.

目的 构建嗜肺军团菌mip基因的真核表达重组质粒pcDNA3.1-mip，并研究其在细胞中的表达。

This study has built a foundation for the development of PAL gene DNA vaccine for Legionella pneumophila.

成功构建了真核表达重组质粒PcDNA3.1-PAL为嗜肺军团菌核酸疫苗的进一步研究奠定了基础。

Methods The hairpin sequences of siRNAs targeting VR1 gene of rat were designed, and two pairs of oligonucleotide sequence were synthesized. The annealed oligonucleotide fragments were cloned into linearized pRNAT-U6.2/Lenti expression vector and identified by PCR and DNA sequencing.

设计靶向大鼠VR1基因的发夹状siRNA，合成两对互补的寡核苷酸序列，退火后克隆到酶切的pRNAT-U6.2/Lenti siRNA载体，通过PCR和DNA测序鉴定重组质粒。

After 3 weeks of continuous observation, we found the infection efficiency still keeping in the range of 80%~90%. Conclusion The lentivirus expression vector for β-catenin was successfully constructed.

在感染慢病毒质粒的人毛囊干细胞，当感染复数为10时，感染后48h的感染效率可达80%以上，感染后3周的感染效率仍维持在80%~90%。

Due to the electrostatic interaction, the positively charged lysozymes can easily assemble onto the negatively charged DNA molecules as dense or sporadic patterns under different experiment condition, and the resulted structures were mainly characterized by atomic force microscopy which is a modern technique.

利用静电相互作用，将带正电的溶菌酶分子疏密可调地组装到了带负电的质粒DNA分子上，通过原子力显微镜等现代技术对溶菌酶在DNA模板上的组装情况进行了研究。

Objective: To study the anti-tumor effect of PTEN eukaryotic expressing plasmid on human bladder cancer xenografts in nude mice.

目的：探讨PTEN真核表达质粒对人膀胱癌裸鼠移植瘤的抑制作用。

Coli might be distributed in all the gene. The intact Hu-P68 cDNA ORF including 10bp upstreams of ATG were obtained by PCR deletion and reconstruction. The resulted fragment were cloned into transfer vector pBacPAK〓 at Bam HI site and lipofectioned into 〓 cells with Bsu 36I digested genomic DNA of insect baculovirus BacPAK 〓.

利用PCR技术删除人P68 cDNA翻译起始位点上游10bp以外的5'UTR，并引入BamHI位点，经酶切和测序验证后，将此改造的完整P68 cDNA基因编码区装入转移载体pBacPAK〓，用脂转染法将此重组质粒与酶切的昆虫杆状病毒BacPAK〓基因组DNA片段导入昆虫细胞〓中，经同源重组获得了再生病毒。

The gene typings of the bacterial from lower respiratory tract secretion and gastric aspirates were taken by plasmial profile analysis,restriction andonuclease fingerprint analysis and Genome Macrorestriction Pulsed Field Gel Electrophoresis in the same phenotype bacteria.

以气管切开或插管患者为研究对象，用防污染标本刷采集标本，由细菌表型分型，直至质粒DNA图谱、酶切图谱、全基因组DNA稀有位点酶切脉冲电场电泳（genome macrorestriction pulsed field gel electrophosresis， GM PFGE）等基因分型方法，前瞻性观察NP的发生情况。

Objective To search for the best approaches to transfect plasmid pEGFP-N1 into human fibroblasts.

目的 采用3种不同的方法将质粒pEGFP-N1转染人成纤维细胞，从中寻找最佳的稳定转染的方法。

Methods:Electroporation was used to transfect LMP gene and the vector into CNE1 cells.

采用电穿孔基因转染技术，将重组EBV-LMP表达质粒转染鼻咽癌细胞株CNE1细胞。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！