质粒

A group of constitutive-expression fusions with different fluorescent strength were obtained.

将重组质粒电转化7653R，获得的转化子发不同强度的绿色荧光。

We observed that FuGene6 regent had the highest transfection efficiency and lowest toxicity to auditory neurons in the three groups. The transfection efficiency was about 3%-10%, variable from experiment to experiment. However, when we used recombinant adenovirus vector to transfer LacZ gene into auditory neurons,β-gal expression was detected in more than 70% of both neurons and gilas at 100M0I viral concentration..

通过对不同转染方法的选择和转染条件的优化，其中FuGene 6介导的质粒转染，其转染效率最高，可达3％-10％左右，但不同批次结果重复性较差，易受不同批次细胞来源动物的个体差异及培养条件等因素的影响。

The results showed that about 3.18 x l0~7/ml of protoplasts were formed when the mycelia were incubated for 1 hour at 28 °C in TSB medium containing 2% glycin, and treated with 2 mg/ml lysozyme.

对东方拟无枝酸菌原生质体制备、再生及质粒DNA转化等条件进行了探索，考察了培养基组分、甘氨酸浓度、溶菌酶浓度等因素对原生质体的制备及其再生的影响。

Cell lines were co-tranfected with bi-plasmids and cultured at 1% O〓 condition, luciferase assay demonstrated that all of the expression systems with different domains could be hypoxia-activated. GAL-CAD achieved more than 100-fold induction in A549 cell and more than 40-fold in HepG2; expression level of the system markedly increased after addition of glycin arm, almost 10-fold enhancement in HepG2 cell. Because basic expression also increased, the induction fold by hypoxia decreased.

双质粒共转染不同细胞株后，在1％O〓环境下培养24h，荧光素酶活性检测结果提示含不同结构域的表达系统均有受缺氧激活的能力，其中GAL-CAD在A549中获得100倍以上的诱导，在HepG2中获得40倍以上的诱导；加入甘氨酸臂后该表达系统的转录活性大为增强，在HepG2细胞中增强了10倍以上，但是由于基础表达也有所增加，受缺氧诱导的倍数下降。

The full hBD2 cDNA was inserted into the multi clone site of a eukaryotic expressive plasmid pcDNA3.1/Myc His and located closely at the upstream of two tag gene (myc and 6 Poly histidines ) so as to construct another recombinant eukaryotic expressive vector of hBD 2 gene: rpcDNA3.1/Myc Hi...

将 h BD- 2的全长 c DNA片段插入编码双重报告基因 Myc和 6个多聚组氨酸的真核表达质粒 pc DNA3.1/Myc- His ，构建出 C端带 Myc和 6× His的 rpc DNA3.1/Myc-His /h BD- 2。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

They also found that "CRISPR interference," as this phenomenon is known, involves the targeting of the incoming plasmid or virus DNA directly.

他们也发现了CRISPR干扰作用，正如被认识的一样，该作用包括间接将进入的质粒或者病毒DNA作为目标。

We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.

我们使用了依赖HCV IRES表达萤火虫荧光素酶的报告基因质粒。

FDR3 was inserted into the yeast expression vector pDBLeu and then was transferred to the yeast strain M3 mediated by PEG/LiAc and by electroporation . In the medium without copper and iron, onlywith ferric citrate , the expression of FDR3 could complement the growth defect of ctrl mutant .

把FDR3基因片段克隆到酵母表达载体pDBLeu上，然后通过电击和乙酸锂转化两种方法将重组质粒导入酿酒酵母Ctrl突变体（M3 mutant）中，采用酵母异源互补法进一步鉴定FDR3基因的功能。

Studied on the antibacterial characterization stability of two lactic acid bacteria which had higher inhibitory activity against indicator organism－ Listerial monocytogenes and Bacillus subtilis in all isolated lactic acid bacteria of the koumiss collected from the herdsman′s home in Inner Mongolia-XiLinGuoLe region.

从内蒙古锡林郭勒盟牧民自制的酸马奶酒中，筛选了两株抑制指示菌－单核细胞增生李斯特氏菌和枯草芽胞杆菌活性最强的乳酸菌，通过质粒的提取和分析，研究其抑菌特性的稳定性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！