质粒

RESULTS: All the four recombinant eucaryotic plasmids were constructed successfully, and HBV core protein expression was confirmed by internal reference for transfection.

结果： 4份重组真核表达质粒均构建成功，转染至HepG2后通过内参照EGFP可鉴定出均有HBV-C蛋白表达。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB expressing in yeast of S. cerevisiae, of which the expressed protein antibacterial activity was verified in preliminary.

目的 构建牛乳铁多肽的真核表达质粒pYES2/Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，并初步检测其不同变异的体外抗菌活性。

The eukaryon expression vector of bFGF is constructed and can be expressed successfully in vitro and in vivo. That is a primary preparation for the research on tissue transplantation and tissue engineering with bFGF gene therapy.

构建了人bFGF真核表达质粒，并可在体内外顺利表达，为进一步组织移植或组织工程基因治疗研究奠定了基础。

OBJECTIVE:To study the effects of eukaryon expressed adiponectin on the glycogen synthesis and glucose oxidation in skeletal muscle cell strain C2C12 myotubes by transfecting plasmids carrying mouse adiponectin.

目的：通过转染带小鼠脂联素基因的质粒，观察真核表达的脂联素对骨骼肌细胞株C2C12肌小管葡萄糖氧化和糖原合成的影响。

Objective To construct recombination eukaryon expression plasmid for human parathyroid hormone gene, assay PTH expression and biological activity after transfection in vitro and evaluate gene therapy effect on hypoparathyroidsm.

目的构建甲状旁腺激素基因的重组真核表达质粒，评价体外转染后PTH基因的表达与生物学活性，同时观察其对甲状旁腺功能减退动物的基因治疗作用。

Results The eukaryon expression system pcDNA3.1/myc-HisC-bFGF could express the target protein bFGF in vitro. The recombinant plasmids, pcDNA3.1/myc-HisC-bFGF and pCD2-VEGF121 were transferred into muscles flap in vivo successfully. The active proteins bFGF and VEGF121were expressed at high levels.

结果 构建的pcDNA3.1/myc-HisC-bFGF真核表达体系成功转染体外培养HeLa细胞，目的基因在mRNA水平和蛋白水平均有表达。pcDNA3.1/myc-HisC-bFGF和pCD2-VEGF121重组质粒分别转染在体肌瓣，获得外源基因高水平表达。

The results revealed that no excision was observed in p3E1.2-CBW-2,but two excisions were observed in p3E1.2-CBW-2,though the frequency was very low(0.00025%).

但HaPLE1构建的质粒具有0.00025%的剪切频率，不过观察到的剪切位点均不是piggyBac转座子的经典剪切位点TTAA。

MATERIALS: One Japan flap-eared rabbit was purchased from the Department of Animal, Kunming Medical College.

材料：日本大耳白兔1只，购于昆明医学院动物科。pCDNA3.1质粒为美国Invitrogen产品。

The method was to set up a freezing time ladder of the mixture of competented cell and plasmid along with a negative sample to research under the same condition of other aspects;The results revealed that the transforming efficiency was the highest at the freezing time of 20 minutes;Compared with the efficiency of other freezing times,the 20 minutes freezin...

在保证其他条件一致的情况下，设置大肠杆菌感受态细胞和质粒混合物的冰浴时间梯度并建立阴性对照进行研究。结果：冰浴20 min时转化效率最高。结论：与其他冰浴时间相比，冰浴20 min时的转化效率最高，实验重复性好，成本低廉，而且操作简便，值得推广使用。

The aim of the research was to confirm the best freezing time of the mixture of competented cell and plasmid in the E.coli DH5α CaCl2 transforming method.

确定大肠杆菌DH5α氯化钙转化法时感受态细胞与质粒混合物的最佳冰浴时间。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！