质粒

Methods: Protein analysis software-PROSPECT was used to divide VP2 into different sections according to hydrophile and antigen character of VP2 amino acids. Correspondent down-lead was designed to extend the section.

以犬细小病毒VP2基因重组质粒为模板，通过蛋白质分析软件PROSPECT分析，根据VP2基因所编码的氨基酸的亲水性和抗原性，把VP2基因分成不同区段，设计相应引物并扩增出相应片段。

Objective: To construct eukaryotic expression vector of adenovirus type 5 fiber gene and investigate its expression in eukaryotic cell .

目的 ：构建腺病毒纤维蛋白基因的真核表达质粒，并检测其在真核细胞中的表达，为腺病毒靶向性载体构建创造条件。

The membrane protein gene of Infectious Bronchitis Virus like was obtained from alpacas for the first time. And it was cloned into the pMD-18T vector.

本试验首次从羊驼体内获取了禽传染性支气管炎样病毒的膜蛋白基因，并克隆到了pMD-18T载体中，获得了膜蛋白基因的克隆质粒。

RESULTS Eleven biotypes,18 antibioticgrams,9 serotypes and 18 plasmidgrams were detected in the 97 strains.

结果97株菌分11个生物型，18个抗菌谱型，9个血清型与18个质粒谱型，但分型率高低不一。

DNA sequencing confirmed that the constructs did have Chlamydial encoding genes in it which were correct in translation frame and sequence.

DNA 序列分析证实重组质粒含有相应的衣原体基因，其读码框架正确。

Second, the genes coding for TPI and ALD were constructed in tandem to form two cistron by altering the base composition and the length of intercistronic region and cloned into 〓 expression system, an active, high-level co-expression was completed successfully.

采用〓启动子成功地获得了TPI—ALD-FBPase三基因活性共表达，构建了三基因共表达的大肠杆菌-蓝藻穿梭表达质粒，并在大肠杆菌中成功地进行了活性表达。

The four proper complete DNA sequences were obtained by splicing the sequenced results with the software SeqMan of the DNA Star. The homology compare of the corresponding nuclear sequences and the cladogram analyze between the obtained virus strain and abroad virus collate strain were measured by Jotun Hein method in software DNA Star.

将MS-1、DL-1、DL-2和DL-3株病毒经组织病料提取总DNA，采用依据标准毒株ADV-G的DNA序列设计并合成的四对引物，经PCR扩增后分别构建重组质粒并测序，将测序结果用DNA Star软件中的SeqMan程序进行拼接，获得四株病毒的近全长DNA序列。

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

Objective To construct a plasmid vector with EGFP reporter gene for functional analysis of enhancers.

目的 构建以EGFP为报告基因的增强子鉴定质粒载体，并对其进行鉴定。

Objective To construct the eucaryotic recombinant plasmid of pYES2/LactoferricinB and express in yeast of S.cerevisiae and preliminary verify the antibacterial activity.

目的构建牛乳铁多肽的真核表达质粒pYES2／Lactoferricin B，实现其在酿酒酵母S.cerevisiae中的表达，初步检测其不同变异的体外抗菌活性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！