质粒

Objective To study the antibioticsusceptibility of 693 strains of bacteria and its relation to the plasmid.

目的 研究693株细菌的药物敏感状况与其质粒的关系。

The resultant recombinant plasmid, terming pMB1, was transformed into E. coil BL21(DE3). IPTG was used to induce the expression of the target protein.

将重组质粒转化到宿主菌BL21 (DE3)中，对重组菌株BL21(pMB1)用IPTG进行诱导表达，并对最佳诱导时间进行了探索。

This P element then transposes from the plasmid to a random chromosomal site.

携带目的基因的P因子可从质粒转座到任意染色体上。

Schistosoma japonicum ; Fatty acid-binding protein ; Recombinant plasmid ; Expression

日本血吸虫；脂肪酸结合蛋白；重组质粒；表达

Objective To clone the sequence of the cDNA of s-lap novel gene coding area and construct its expression vector.

目的：克隆新基因s-lap编码区序列并构建其重组表达质粒。

Result: Restriction enzyme analysis proved that cloning vector pEGFP-RECK was successfully constructed, and the sequencing results of it was in accord with expected. By transfecting the pEGFP-RECK vector into SHG44 cells, we could see the protein locate mainly in cell plasm.

结果：所得RECK基因融合蛋白表达载体序列与预期相符。pEGFP-RECK表达质粒转染SHG44细胞后，荧光信号主要集中在与细胞质有关的部位。

The total RNA of viruses isolated was extracted from the Hantavirus-infected Vero-E6 cells and the S-segment cDNA of Hantavirus was obtained by RT-PCR. This gene fragment was then cloned into plasmid vector pUCm-T and sequenced afterwards by using the DNA STAR software for comparison.

提取病毒总RNA，应用逆转录聚合酶链反应扩增病毒S基因全片段，克隆入质粒载体，进行核苷酸序列测定及分析，应用DNA STAR软件分析比较，确定病毒型别。

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods 14 mutanted vpr fragments selected from Chines patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products purified , double-cut by HindⅢ and BamH and the cut products legated and were into competent cells JM109. The 14 reconstructed plasmids electronically transfected into hela cells, and established cells with pcDNA vprwt 、pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell.

将14个带有HIV-1 vpr基因片段的pcDNA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vp r基因转染细胞、突变株 vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经RT-PCR检测目的基因转染成功后，经Pi染色用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

Methods:The producing ESBLs bacteria were betected by double-disk synergy test with indicators of Aztreonam,Cefotaxime,Ceftriaxone and Ceftazidime.

由于ESBLs是质粒介导的，可在不同细菌之间传播，容易引起院内的暴发流行，给抗感染治疗和院内感染带来极大危害。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！