质粒

Kanic acid was injected into guinea pigs' cochleae and the excitotoxicity model was established. After a week the recombinant plasmid was transferred into SGCs of guinea pigs' cochlea treated with HAT. The following week the expression of NT-3 was examined by the immunohistochemical method, and the morphology of SGNs was observed under the electronic microscope after 4 weeks, in the mean time the changes of auditory brain-stein response were examined.

通过耳蜗灌注海人酸(kainic acid， KA)建立豚鼠耳蜗兴奋性损伤模型，在给KA 1周后利用HAT携带重组质粒进行耳蜗灌注以转染耳蜗螺旋神经节细胞，免疫组化法观察转染后1周NT-3的表达及4周后电镜下螺旋神经节细胞形态学变化，同时观察对听觉脑干诱发电位(auditory brain-stem response， ABR)的影响。

A modified method for isolating mitochondrial plasmid-like DNA from etiolated plant seedlings was set up at first.

本文在文献报道的实验方法基础上，建立了一种简便、快捷、有效的提取植物线粒体DNA类质粒的方法。

The two reconstructed plasmids were packed to infective virus particles in incasing cells PT67 with a tite of about 5. 8×10〓-3. 4×10〓cfu/ml.

2两种重组表达质粒在PT67细胞中包装成具有感染能力的病毒颗粒，产生病毒滴度在5.8×10〓-3.4×10〓cfu／ml之间。

The results indicated that the insets were all same in size and a few were different in sequence.

将200bp PCR产物进行了分离、纯化和克隆，并对随机选取的22个重组克隆质粒的酶切和PCR-SSCP分析后，实验结果显示，这些克隆的插入片段长度一致，其中部分片段间具有不同的碱基构成。

The infected cells were cultured for 72h, then lysed and determined for luciferase content.

结果 HIV-1的B/C重组亚型和C亚型Envelope基因真核表达质粒分别与pNL4-3。

The bacteria lyses quickly after immunization, it can express HBsAg/SS protein, produce SS antibody which neutralize the inhibition of SS, so the SS gene vaccine can promote growth.

该疫苗进入体内能迅速裂解释放质粒，表达乙肝表面抗原／生长抑素的融合蛋白，刺激机体产生SS抗体以中和SS，解除SS对生长的抑制作用，促进动物生长。

Necropsies were performed at 21 days post-challenge, the lymph nodes in the nonvaccinated but challenged pigs were larger than those of vaccinated pigs. The PCV2 genomic copy loads in lymph nodes were reduced in vaccinated pigs. Moderate amounts of PCV2 antigen were detected in most lymphoid tissues of nonvaccinated pigs. These data indicated that when given intramuscularly in pigs, the chimeric PCV1-2 live virus and recombinant plasmid induces protective immunity against PCV2 infection and could potentially serve as an effective vaccine.

攻毒后21天攻毒对照组猪淋巴结比免疫组猪显著肿大，免疫组猪血清、淋巴结中PCV2病毒载量低于对照组，攻毒对照组猪淋巴结中PCV2抗原含量高于免疫组，这些结果表明嵌合型猪圆环病毒PCV1-2及真核表达质粒肌肉注射免疫商品猪后，对PCV2感染能产生保护性免疫应答，有可能成为候选疫苗。

The expression plasmid of hIL-3 gene nuder the COntrol ...

构建的PL启动子控制下的hIL－3cDNA表达质粒，转入大肠杆菌Tap106，经42℃热诱导，获得hIL－3的表达产物。

The expression plasmid of hIL-3 gene nuder the CO...

构建的PL启动子控制下的hIL－3cDNA表达质粒，转入大肠杆菌Tap106，经42℃热诱导，获得hIL－3的表达产物。

Methods: The medicinal plant Rheum wittrochii Lundstr., was genetically with Ri plasmid harbouring in Agrobacterium rhizogenes 9402 strain to induce hairy roots; transformed the opines from the hairy roots were assayed by paper electrophoresis; the contents of free anthroquinones such as aloe-emodin, rhein, chrysophanol, emodin, physcion, 8-O-methylchrysophanol from the hairy roots were quantitatively determined by HPLC technique.

用发根农杆菌Agrobacterium rhizogenes 9402 所含Ri质粒遗传转化药用植物天山大黄以诱导发根用纸电泳法检测发根中冠瘿碱的存在；用高效液相色谱法测定发根中芦荟大黄素、大黄酸、大黄酚、大黄素、大黄素甲醚、大黄酚-8-甲醚等游离蒽醌化合物的含量。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！