质粒

In this study we constructed the recombinant pcDNA-HA-rpb in E.coli cells and we expressed the three polymerase II subunits; Rpb5, Rpb6 and Rpb7 as HA-tagged polypeptides by transfection in 293T mammalian cells, as well as untagged nuclearβ-actin.

在这项研究中，我们在大肠杆菌中构建了pcDNA-HA-rpb重组质粒，表达三个聚合酶II亚基： Rpb5 ， Rpb6和Rpb7，通过转染哺乳动物细胞293T，从而表达带HA标签的多肽，同时转染不带标签的β-actin。

Molecular analysis and disease-resistance test The regenerated kanamycin resistant plants were analyzed by PCR andPCR-Southern reaction with the untransformed plants as negative control, plasmidDNA as positive control.

转基因植株分子检测及抗病性鉴定以未转化植株为阴性对照，质粒DNA为阳性对照，nptⅡ基因和chit42基因的特异引物进行PCR和PCR-Southern检测。

The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

为了克隆禽腺联病毒(Avian adeno-associated virus， AAAV)全基因组用于构建基因转移载体研究，以鸡胚致死孤儿病毒作为辅助病毒与AAAV共接种SPF鸡胚进行AAAV的增殖，将AAAV约4.7 kb双链基因组DNA与pCR2.1载体连接，构建了含AAAV全基因组的重组质粒pAAAV并进行了测序。

The remaining eighteen pigs, served as three viral administered groups, underwent coronary artery ligation and received intramyocardial viral injection. rAAV- GFP group (n=6), rAAV-CD151 group (n=6) and rAAV-antiCD151 group (n=6) animals respectively received direct intra- myocardial injection of rAAV-GFP, rAAV-CD151 and rAAV-antiCD151 1×10~(12 viron particles per pig, at 10 sites correspondingly.

采用三质粒共转染法包装CD151、antiCD151和GFP重组腺相关病毒(recombinant adeno-associated virus，rAAV)。2.1月龄小型猪22头，随机分为4组，分别为正常对照组4头(不予冠脉结扎和注射病毒，正常喂养)，rAAV-GFP组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-GFP病毒，rAAV-CD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12viron particles的rAAV-CD151病毒，rAAV-antiCD151组6头结扎冠状动脉左前降支，心肌内分10点注射1×10~(12vironparticles的rAAV-antiCD151病毒。

The results showed that the reporter plasmid could be used to identify the genes which positively regulate the expression of hrpX in Xanthomonas campestris.

实验结果表明该报告质粒可用于筛选鉴定正向调控野油菜黄单胞菌hrpX表达的基因。

Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

Objective To develop a PCR-based method for rapid and effective screening of arrayed plasmid cDNA library.

目的 建立一种快速、有效地筛选质粒cDNA文库的方法―PCR法。

The expression of COX-2 was assayed by RT-PCR and Western blot after HepG2 and BEL7402 cells were transfected with liposomes for 24, 48, 72 and 96 hours.

质粒在HepG2细胞和BEL7402细胞中的转染率分别为60%，54%。

Pollen-DNA mixture was treated by ultrasound with the intensity of 400W, and besmeared on the filament to make zea mays L be fertilized.

用400W超声功率处理花粉-质粒DNA混合物，将经过处理的花粉涂于花丝上进行授粉。

All mice were immunized for three times with an interval of two weeks. The mice were challenged with（40±1） cercariae of S.

每鼠肌肉注射相应的纯化质粒DNA 100 μg，每隔3周免疫1次，共3次。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！