质粒

Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification.

用能扩增长片段的高保真耐热 DNA聚合酶扩增全长的质粒DNA，直接转化大肠杆菌。

The subcellular localization of TIAR full length and truncated mutants were observed by using fluorescent microscopy.

酶切鉴定及测序结果显示TIAR全长及缺失体的pEGFP-C3重组表达质粒构建成功。

We prepared critical size segmental bone defects in rat tibiae and transplanted GAM consisting of boine atelocollagen and expression plasmid ector (bmp2), which encodes human BMP2, with or without CaP.

我们在大鼠胫骨上做了临界大小的节段性骨缺损，并移植上包含牛次发育胶原、表达bmp2质粒的GAM，后者编码人BMP2，不管有没有磷酸钙沉淀物的存在。

An in vitro transcription recombinant plasmid 5T1d was obtained after subcloning the target gene of clone 5T1d,which contained mutant HCV 5 non-coding region cDNA,into a transcriptive vector pCDNA Ⅰ.

采用含HCV5非编码区(5NCR)缺失突变的重组质粒5T1d，将其目的基因亚克隆到体外转录载体pCDNAⅠ多克隆位点上，获得转录重组体5T1d。

One positive clone was obtained from the libraries with the tributyrin flat and ELISA sieve method. Analyzed the sequencing result, the positive clone include the Open Read Frame of low-temperature alkaline lipase gene.

序列测定分析表明，此重组质粒包含有长度为此95lbp的低温脂肪酶基因的完整的开放读码框架和上游调控序列。

After a single intramuscular injection of plasmid hlL-6 Cdna on 6.5 Gy-irradiated mice, the IL-6 level showed less changes in unirradiated mice.

经6.5 Gy照射的小鼠一次肌内注射表达IL-6的质粒DNA后，用ELISA方法检测血浆IL-6水平，以蛋白表达量评价基因转移效率。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型心理病毒 PrM-E基因重组质粒DNA具有一定的免疫原性。

The recombinant plasmid DNA of PrM-E gene but not unmethylated CpG was capable of inducting antibodies against D2-43 virus.

我国登革2型病毒PrM-E基因重组质粒DNA具有一定的免疫原性。

Methods The recombinant plasmid of CPB, pET-21a-CPB, was constructed and transformed into E. coli BL21(DE3). The expression was induced in 25℃and 37℃, respectively. The obtained inclusion bodies were dissolved in different denaturing solution systems. The protein concentration was determined in order to indicate the solubility of CPB inclusion bodies in the denaturing solution. And probable mechanism for the solubility differences was illuminated by the analysis of unreduced SDS-PAGE.

方法构建CPB重组质粒pET-21a-CPB，将其导入表达菌株BL21（DE3）中，分别在25 ℃和37℃进行诱导表达；所得包涵体分别用不同浓度尿素添加不同还原剂溶解，以溶液中的蛋白质浓度判定其溶解性，利用非还原SDS-PAGE分析其溶解性提高的原因。

It was found that the unstimulated human peripheral blood leukocytes only expressed a low level of MEFV mRNA.

RT-PCR扩增后的MEFV基因片段成功导入质粒载体，并且可以在大肠杆菌中培养生长。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！