质粒

PCC7120 could remain segregative stability of recombinant plasmid pDC-TNF.

影印法证实转TNF -α基因鱼腥藻 712 0能保持质粒分配稳定性。

The important role of hydroxycinnamic acids,i.e.,caffeic acid, chlorogenic acid,sinapic acid,ferulic acid,3-hydroxycinnamic acid (3-HCA) and 4-hydroxycinnamic acid(4-HCA) as the pBR322 plasmid DNA-Cleaving agents in the presence of Cu ions was investigated.

我们研究了羟基肉桂酸衍生物即咖啡酸、绿原酸、芥子酸、阿魏酸、3-羟基肉桂酸(3-HCA)和4-羟基肉桂酸(4-HCA)在Cu存在下诱导pBR322质粒DNA链断裂损伤情况，结果发现HCAs的促氧化活性与它们的分子结构有密切的关系，具有邻二羟基结构和邻二甲氧基羟基结构的化合物表现出了更高的促氧化活性，其中CaA的活性最高。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

设计合成一对特异引物，分别带有 SnaBⅠ和 NotⅠ的限制性内切酶的识别位点，以 pPIC9K-NGF重组质粒为模板，用 PCR 方法扩增 NGF cDNA 片段。

The immuno-protective activity of the vaccine was determined by CTL induction and tumor growth inhibition in vaccine-immunized syngenic mice.

以重组质粒免疫C5 7BL/ 6小鼠，检测诱发的特异性CTL活性；将免疫后的小鼠用TC - 1肿瘤细胞攻击，观察免疫保护效果。

In PCR detection systems, the DNA of recombinant plamid of TCK was used for safely positive reference and genomic DNA of the healthy wheat and rye was used for negative reference.

选择重组质粒作为检测中的阳性对照，健康小麦和健康黑麦基因组DNA作为阴性对照。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

A set of spectific primers was syn thesized according to HBV DNA sequence of Chinese strain, the whole X region was amplified by PCR method from the serum of 9 patients with chronic HBV infection , and then the PCR products were subcloned into pGEM Teasy vectors. Clones were randomly selected to be sequenced. Comparison of the cloned sequence was made to find the difference. After being compared, each sequence of selected clones is o f difference. The point mutation scattered through X region. Deletion mutations were detected in 19 clones of 37(51.4%), which caused different carboxyl endings of X protein. There is a hot region (after 123 aa code) where deletion mutation frequently happens.

以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，自9例慢性HBV感染患者血清中扩增HBV X基因，克隆入pGEM Teasy质粒，随机挑选克隆进行D NA测序以确定病毒的变异程度。37例测序结果提示来源于不同患者HBV X基因序列高度保守，但每个序列均不一致。X区除了存在广泛的碱基点替换突变外，序列的缺失突变占测序克隆总数的51.4％（19／37）；氨基酸缺失及移框突变多发生于123位氨基酸残基之后，可导致X蛋白多种羧基端形式。

During the past 3 years, 2 independent experiments of sperm-mediated gene transfer in domestic silkworms were carried out respectively in 2000 and 2002 with the method direct injection of copulation thecae.

在同源重组绿色荧光蛋白表达载体pG350的基础上，以pMD18-T为质粒载体，用egfp基因替换gfp基因，构建了家蚕核型多角体病毒早期即刻蛋白基因启动子BmNPV-IE控制下的增强型绿色荧光蛋白表达载体pMD-Fib-IE-EGFP。

As for the molecular genetics of plasmids in thermophilic bacteria, only preliminary observation of very few phenomena has been reported so far.

在高温细菌质粒的分子遗传学方面，已发表的少量文献仅限于对个别现象所作的初步描述。

On other hand, specific fragments containing thermostable DNA polymerase gene were amplified from the genomic DNA of some strains of thermophilic bacteria isolated from Yunnan hot springs, and recombinant plasmids were constructed.

同时，我们还利用特异性的热稳定DNA聚合酶基因的扩增引物，从生长在云南温泉的嗜热菌总DNA中筛选出了能被此类引物扩增的特异性片段，并构建了相应的重组质粒。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！