质粒

After the recombinant plasmid was certified by DNA sequencing,the conservative region of Ki67 gene was inserted into pEGFP vector reversedly.

方法用基因重组方法将人Ki67基因cDNA保守序列反向克隆到真核表达质粒pEGFP-C1中，构建人反义Ki67核酸载体。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，将溶酶体的靶向信号肽KFERQ连接在RTA的羧基端；将构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受态大肠杆菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Methods: A lysosome-targeted singal KFERQ was added to the C terminus of rRTA by DNA recombinant technology. A pKK223.3 expression system in E. coli was used to produce recombinant ricine A chain and rRTA-KFERQ. Recombinant proteins were purified by affinity chromatography using Blue-Sepharose 6B.

用DNA重组技术，將溶酶体的靶向信號肽KFERQ连接在RTA的羧基端；將构建好的重组质粒pKK223.3-RTA和pKK223.3-RTA-KFERQ转化感受態大肠桿菌JM109，经IPTG诱导表达RTA和RTA-KFERQ蛋白；重组蛋白质用Blue-Sepharose6B亲和柱纯化，以MTT法分别测定纯化后的RTA与RTA-KFERQ蛋白对体外培养的HEPG2、Hela、A5493种细胞的毒性作用。

Four individual of cross bred cancer cellular MdrA, MdrB, MdrC and MdrD ofexudation against MDR 1 monoclonal antibodies were obtained by injection Balb/Cmice with pcDNA3/P-gp plasmid enwrapped by span and glycerin and followed byamalgamation, riddling and cloning.

将构建的表达质粒pcDNA3/P-gp，佐以司苯、甘油免疫小鼠，通过融合、筛选、克隆，获得了4株分泌抗MDR 1 McAb杂交瘤细胞株MdrA、MdrB、MdrC、MdrD。

The multicopy gene saccharomycetes expressed recombinant plasmid pAOnsGH, the experiment shows, has very high expression activity to make the engineering saccharomycete product possess low production cost and high efficiency. Trial raising shows that feed with the said saccharomycetes added can raise the growth speed, disease resistance and output of the raised animals.

多拷贝基因酵母菌表达重组质粒pAOnsGH，经实验证明具有很高的表达活性，使本工程菌制品实现了生产成本低、效率高的效果，经多次水产养殖试验及养鸡试验，使用添加本酵母菌制品的饲料饲，喂养殖的动物可提高其生长速度和抗病害能力，提高单位养殖产量20％以上，增产的收入大于本制品成本投入的五倍以上。

Intragastric administration of attenuated salmonellae carrying HGF and GFP was performed on rats, each one with 0.2 mL, and once every two days for totally 3 times.

将携带HGF和GFP基因真核表达质粒的减毒沙门氏菌分别给予大鼠灌胃，每只0.2 mL，隔日1次，共3次。

The effects of plasmid pcDNA3 on Ca (superscript 2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle was investigated.

在大鼠肢体缺血模型上观察了质粒pcDNA3月对缺血骨骼肌肌浆网Ca(上标 2+)转运的影响。

The plasmid pDM803, carrying the bar gene, conferring resistance to the herbicide, and the screenable uidA gene, was transferred into wheat by particle bombardment.

在此基础上，将含有bar基因和GUS报告基因的质粒pDM803用基因枪法转化小麦，获得可育的转基因小麦植株，从而建立了适于中国春性小麦基因型的基因枪转化体系。

Objective: The prospective investigation of lymph secluding metastasis of T2-4 N0-phase in head-neck squamous cell carcinoma of small sample, and made prepare for constructiving plasmids.

目的：通过检测T2~4 N0头颈部鳞状细胞癌培养的肿瘤细胞及新鲜活检组织中VEGF-A、C及VEGFR-3的表达情况及构建质粒，探讨是否可以通过组织小样本前瞻性研究了解T2~4 N0患者淋巴管受累及局部淋巴转移情况并为制备VEGFR-3抗体作好准备。

The strain carrying pLEB590 was shown to be able to grow in medium containing a maximum of 550 IU Nisin/ml, and the host strain could maintain the segregational stability of pLEB590 under selective conditions.

携带载体pLEB590的菌株可以耐受最大为550 IU /ml 的Nisin浓度，并且在Nisin选择压力下能够保持外源质粒的遗传稳定性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！