质粒

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

Results Multicopy recombinant plasmids pDGXHP2.0-2M Fα-HBsAg and pDGXHP2.0-4MFα-HBsAg were constructed. Three recombinant Hansenula polymorpha strains for secretory expression of HBsAg were screened.

结果 构建了HBsAg汉逊酵母多拷贝表达质粒pDGXHP2.0-2MFα-HBsAg和pDGXHP2.0-4MFα-HBsAg，筛选获得了分泌表达HBsAg的汉逊酵母HP/2MFα-HBsAg和HP/4MFα-HBsAg。

The fusion gene was inserted downstream to the MOX promoter of Hansenula polymorpha shuttle plasmid pDGXHP2.0, and the constructed multicopy secretory recombinant plasmid was transformed to Hansenula polymorpha ATCC34438 Ura3(superscript - by electroporation.

将融合基因插入汉逊酵母穿梭质粒pDGXHP2.0的甲醇氧化酶基因启动子下游，构建成多拷贝分泌型重组表达载体。

But there is no report about the research using YC2.1 in Schizosaccharomyces pombe.

本文首先构建了含YC2.1基因的粟酒裂殖酵母表达质粒pESP-YC2.1。

In high dentity fermentation, the cellular respiration, energy metabolism, highest bacterial density and dry bacteria weight of the G830 strain were markedly better than control strains PA1 and BL21, The expression of recombinant prolyl endopeptidase in G830 and PA1 under the above condition was high and stable. Their growth situation and fermentation parameters were similar with their parental strains without plasmids, and resided plasmids maintained stably in those strains.

在高密度发酵条件下，G830的细胞呼吸强度、能量代谢、最高菌密度和细胞干重，均明显优于对照菌株PA1和BL21；重组蛋白脯氨醯内肽酶在G830和PA1中获得稳定高表达；重组菌生长状况及发酵指标均与空宿主菌基本一致且表达质粒能维持较好的稳定性。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石。。。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The tryptophan auxotrophs and the kanamycin resistant prototrophic revertants have been proved to contain a copy of Tn5 inserted in the chromosome by Southern Blot analysis with DIG-labeled 3. 5 kb HindⅢ fragment of pkan2 as the Tn5 probe. Four typies of prototrophic revertants could be found by the above assay.

用Tn5探针进行的Southern分子杂交证明Tn5在色氨酸营养缺陷型菌株及部分Km抗性原养回复突变型菌株中均插入在染色体DNA上，并且对菌株本身具有的内源性质粒的数目及大小没有带来影响。

AIM: To detect the stability of the recombinant adenovirus plasmid containing major outer membrane protein gene from Chlamydia psittaci in HEK293 cells, to evaluate whether cross reactivity exists or not between recombinant adenovirus and positive serum of Egg Drop Symdrome, and to evaluate whether SPF chicks vaccinated with the recombinant adenovirus were protected or not when challenged with virulent CpL strain.

目的： 检测重组腺病毒质粒在HEK293细胞中的稳定性；重组腺病毒是否与减蛋综合征阳性血清发生中和反应，以及用重组腺病毒免疫SPF小鸡是否产生保护。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！