质粒

The nucleotide sequence of both termini has been determined, the terminal sequence of the PfDNV DNA has inverted repeats of 201 nucleotides and the terminal 122 nucleotides are palindromic.

利用构建的黑胸大蠊浓核病毒全基因组重组质粒(PfDNV-pUC119)以及一系列完整缺失克隆，通过全自动测序方法完成了PfDNV两末端各728 bp 和730 bp 核苷酸序列的测定。

The recombinant vector was constructed by subcloning SV40LT gene (2.1 Kb) from SV40LT gene-carrying plasmid pLLTSN into the BamH I and Xho I sites of pantropic retroviral expression vector pLXRN.

从携带SV40LT基因的质粒pLLTSN中高保真PCR扩增出2.1 Kb的SV40LT基因，将其插入到泛嗜性逆转录病毒载体pLXRN的BamHⅠ和XhoⅠ位点之间，构建重组载体pLXRN-SV40LT。

The expression of sTNFRⅠin transfected pericementum cell culture supernatant was higher than the contrast groups.

质粒PcDNA3.1-sTNFRⅠ可以在牙周膜细胞中高效表达，并且可以抑制肿瘤坏死因子的活性。

The soluble human tumor necrosis factor ; human tumor necrosis factor ; plasmid ; pericementum cell ; tranfected

可溶性肿瘤坏死因子；肿瘤坏死因子；质粒；牙周膜细胞；转染

Objective:To recombine the plasmid PcDNA3.1-sTNFRⅠ,and detect the expression of recombinant plasmid in pericementum cell,and evaluate the bioactivity of expressed sTNFRⅠ.

目的：构建真核表达质粒PcDNA3.1-sTNFRⅠ，并转染人牙周膜细胞观察是否有目的产物的表达，研究其是否具有生物学活性，为进一步研究其在牙周治疗中的作用提供实验基础。

Recombining the plasmid PcDNA3.1-sTNFRⅠ.sTNFRⅠin cell culture supernatant was detected by ELISA and sTNFRⅠblockage of TNFαin pericementum cell by MTT assay.

RT-PCR方法筛选目的基因，并构建质粒；ELISA方法检测牙周膜细胞转染PcDNA3.1-sTNFRⅠ后培养上清中目的基因的表达；MTT检测目的产物是否在牙周膜细胞中有抑制TNF-α的作用。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片，在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

This study provides a new system to produce phytase with a potential for an application in related industry.

本研究提出的pEGFP-N3- aPPA2重组质粒构建和在哺乳类COS7细胞表达体系为植酸酶生产提供了新的技术线路。

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料，以激发标签为技术平台，构建了拟南芥的功能获得突变体库，从中筛选具有抗病、抗逆表型的突变体，克隆基因并进行基因的功能研究，取得的主要结果如下：(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株，以抗除草剂的Bar基因为筛选标记，获得了约有12000个独立转化株系的突变体库，并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！