质粒

While AcMNPV and BmNPV had no stimulatory effect in nonsensitive cell lines BmNPV hr3 has been demonstrated to increase the transcriptional activity of cislinked hel510 promoter. In this study, Sf-21, Bm-N and Hi-5 cell lines were transfected with pBmhel510luc-hr3down, and then were infected with each of AcMNPV, BmNPV and HyNPV. The results indicated that viral factors could interact with hr3 to significantly copromote the transcriptional level of hel510 promoter.

BmNPV hr3已被证明具有增强子功能，将hr3与hel510启动子顺式连接的质粒pBmhel510luc-hr3down转染Sf-21、Bm-N和Hi-5细胞后，再用上述三种病毒分别感染每一种细胞，hel510启动子的转录活性得到显著提高，表明病毒因子能够识别hr3，二者通过相互作用，共同增强解旋酶基因启动子的转录水平。

Objective To study the humoral and cellular immune responsesinduced by four DNA vaccines of CVB3 structural and nonstructural proteins.

目的 制备4种柯萨奇B3病毒（CVB3）结构和非结构蛋白重组质粒DNA疫苗，并探讨其诱导机体产生体液和细胞免疫应答的效果。

Although octopine synthase and mannopine synthase genes initally resident on Ti plasmid of Agrobacterium tumefaciens, they possess all of the sequence elements required for transcription in plants.

甘露碱合成酶基因启动子和章鱼碱合成酶基因启动子虽然来源于农杆菌Ti质粒，但却具有植物启动子的特性，是目前使用广泛的两个组成型启动子。

Results of studies displayed: 1. We first constructed successfully cDNA expression library of the Oncosphere development stage of Teania solium.

结果表明： 1。利用质粒表达载体pSPORT 1首次成功构建了猪带绦虫六钩蚴cDNA表达文库。

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,ChinaAbstract: To obtain the hybridoma cell line specific to 45W-4BX antigen of Taenia solium oncosphere,the recombinant plasmid pGEX-4BX containing TSO45W-4BX gene was highly expressed in Escherichia coli induced with IPTG.

为获得针对猪带绦虫六钩蚴45W-4BX抗原的杂交瘤细胞株，用IPTG对TSO45W-4BX的重组质粒pGEX-4BX进行了诱导表达，并采用Sepharose-4B层析技术对表达产物进行纯化，运用SDS-PAGE和Western-blot分别对其纯度和活性进行了检测。

The Taenia solium oncosphere 18 gene was cloned and reconstructed into expression vector pYA3341.The recombinant plasmid pYA3341-TSOL18 was finally electro-transformed into an attenuated S.typhimurium definitive host strain of X4500 and the recombinant strain of X4500 (pYA3341-TSOL18) was identified its immunogenicity of expression protein, stability, growth curve, safety in vitro and immunity evaluation by animal experiment in mouse.

克隆并改造TSOL18基因，构建重组质粒pYA3341-TSOL18，电转入鼠伤寒沙门氏菌终宿主菌株X4550，体外鉴定重组菌X4550(pYA3341-TSOL18)表达蛋白的免疫原性、稳定性、生长曲线、安全性和小鼠免疫试验进行评价。

The results of sequence analysis shown that the ORF of MP gene is 804bp.encode 268 amino acids .

将其克隆到pMD-18-T vector上，得到含有完整MP基因的重组质粒pTMP-D。

Digested by endonuclease, the linear plasmid was injected into the copulatory pouch of the female moth and the sperm reservoir of the male moth. Let them mate with the normal moth and oviposit.

将打靶质粒酶切线性化后，分别注入到雌蛾交配囊和雄蛾贮精囊，与正常蛾交配产卵，分析外源基因在当代的情况。

Methods : OMP2b protein gene fragment was amplified from genomic DNA of Brucella ovis in XinJiang through PCR.

采用PCR扩增技术，从新疆绵羊种布鲁氏菌基因组DNA中扩增出OMP2b基因片段，将该片段克隆于原核表达载体PE-28a，构建成重组质粒，IPTG诱导表达，SDS-PAGE检测有无蛋白的表达。

The successes in connecting SDION with pGFP-C2 plasmid by means of oxidoreduction reaction and in transferring pGFP-C2 gene into human bladder cancer BIU-87 cells in vitro provided the experimental evidence for the feasibility of SDION used as a novel gene carrier.

SDION可通过氧化还原反应与GFP质粒相连，在体外可将GFP基因成功转染入人膀胱癌BIU-87细胞。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！