质粒

Specific primers were designed according to gene NHX1 of Limonium gmelinii published on NCBI. Then, the gene was cloned by means of RT-PCR. Eukaryotic expression vector pCAM-BIA1301-NHX1 had been constructed, then transformed into tomato by Agrobacterium-mediated transformation method. Transgenic tomatos were obtained.

根据NCBI公布的大叶补血草NHX1基因序列设计特异性引物，经RT-PCR方法克隆得到大叶补血草液泡膜Na/H逆向转运蛋白基因NHX1，构建植物融合表达载体pCAMBIA1301-NHX1，重组质粒通过农杆菌介导转化番茄，经PCR和RT-PCR鉴定，获得转基因番茄植株。

The expression of cloned cold-adapted lipase gene lipA in Pichia pastoris GS115 with the pPIC9k as the expressive vector was performed according to the methods described in the manual, version 3.0, of the pPIC9K identified the lip gene cloned here was a functional lipase gene.

以pPIC9K为表达载体，以GS115为表达宿主菌，成功构建重组表达质粒pPIC9K-lipA，经甲醇诱导表达，能够分泌有脂肪酶活性的表达产物，因此证明本研究所克隆的脂肪酶基因lipA确实为脂肪酶开放阅读框。

We first constructed the CAT and Luc reporter plasmid deleting CRE or AP-1 site or both.

首先构建了CRE和AP-1位点系列缺失的CAT和Luc报告基因质粒。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

Great quantities of early or late Apis mellifera ligustica eggs were electroporated with green fluorescent protein gene on the optimized conditions.

实验用质粒pEGFP-N1对意大利蜜蜂卵电转导条件优化进行系统研究，然后利用所得到的电穿孔优化条件，对大量意大利蜜蜂早、晚期卵进行绿色荧光蛋白基因转移研究。

New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis.

qnrC，一种从奇异变形杆菌分离得到的新型的质粒介导的喹诺酮抵抗基因

Conclusions】 It is suggested intramuscular injection is an effective immune route. Mice inoculated with coding different stage gene recombinants alone or mixedly could all induce increased humoral and cellular immune response, and NK cell activity.

提示肌肉注射为一有效的DNA疫苗免疫途径，采用编码恶性疟原虫有性期阶段或无性红细胞阶段的重组质粒单独或两者混合免疫小鼠，均能诱导明显的体液免疫反应、细胞免疫反应和NK细胞杀伤活性。

The recombinant pcDNA3-EBA175/HRPⅡ and pcDNA3-Pfs25 were injected alone or mixedly into mice by intramuscular way respectively. The kinetic changes of IgG antibody value, the splenic lymphocyte proliferation, the ratio of CD4+/CD8+ subgroups and NK cell killing activity in each group were observed.

将恶性疟原虫FCC-1/HN株重组质粒pc DNA3-Pfs25及pcDNA3-EBA175/HRPⅡ经骨骼肌途径分别单独注射或两者混合注射免疫BALB/c小鼠，观察免疫后不同时间点血清中IgG抗体滴度、脾淋巴细胞增殖反应、CD4+/CD8+ T细胞亚群比值和NK细胞杀伤活性的变化。

Plasmid pO26-CRL is nonconjugative but is mobilizable.

质粒pO26-CRL是非结合转移类的，但它具有可移动性。

Methods: The human KDR promoter (-225 bp~+127 bp) was cloned by PCR. Subsequently, a recombinant adenoviral plasmid carrying KDR promoter for the HSV-tk gene was constructed with the AdEasy system. As a control, the plasmid pAdCMV-tk carrying CMV promoter for the HSV-tk gene was also constructed. 293 packaging cells were transfected by both newly-constructed plasmids and the infectious viruses AdKDR-tk and AdCMV-tk were generated. Then the KDR-producing cells and the KDR nonproducing cells (HepG2) were infected with AdKDR-tkand AdCMV-tk, followed by ganciclovir administration.

应用PCR克隆出人KDR基因启动子序列－225bp~+127bp，以AdEasy system为载体，构建携带受KDR启动子或CMV启动子调控tk基因表达的2种重组腺病毒质粒pAdKDR-tk和pAdCMV-tk，在293细胞中包装、扩增后，体外感染表达KDR的脐静脉血管内皮细胞系HUVEC和不表达KDR的肝癌细胞系HepG2，并给予不同浓度的丙氧鸟苷处理，5d后收集存活细胞并计数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失