质粒

An immunogenically active component of West Nile virus or plasmid DNA, an adjuvant such as a metabolizable oil, and a pharmacologically acceptable carrier are formulated into an immunizing vaccine.

将一种西尼罗河病毒或质粒DNA的免疫原性活性成分、一种诸如可代谢油的佐剂和一种药学可接受载体共同配制成一种免疫疫苗。

Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. At the 14 days and 28days after second operation, we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular were calculated by computer.

用注射式加压转染的方式对兔动脉壁转染pcDNA3.1/hTM质粒，再制造动脉损伤-阻滞模型，于术后14天、28天用彩色多普勒观察活体吻合口内径和血流流速；再做病理切片Verhoeff染色，观察血管内膜增生的程度、部位，计算血管内膜面积、中膜面积和血管狭窄率。

BALB/c mice were injected with the recombinant pCMV-ME plasmid DNA contained PrM-E gene interdermally and intramuscularly.

在证明其可在哺乳动物细胞中高效表达的基础上，进一步用重组质粒DNA免疫小鼠。

BALB/c mice were injected with the recombinant pCMV-ME plasmid DNA contained PrM-E gene interdermally and intramuscularly.

在证明其可在哺乳动物男性细胞中高效表达的基础上，进一步用重组质粒DNA免疫小鼠。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Immunofluorescence staining shows that, the CaN Aβ protein expression of intrapericardial injection group decreased in some myocytes under epicardium, while other groups show no decreased fluorescence.

三在体心肌CaN AβsiRNA表达质粒转染后，转染各组与心肌肥大模型组CaN AβmRNA水平比较无差异(P＞0.05)，但免疫荧光结果显示心包腔内途径CaN Aβ干扰组较心肌肥大模型组心外膜下部分心肌荧光减弱。

In this part we decided to evaluate the efficacy of this mIL-4RA on asthmatic airway inflammation and Th1/Th2 cell disfunction through intratracheal administration.

目的：通过气管内滴注mIL-4RA的真核表达质粒，观察对哮喘小鼠气道炎症及Th失衡的干预作用。

The experimntal results showed that the recombinant plasmid could be expressed in the COS-7 cells with a typical T-helper 1-dominanted immune response as demonstrated by IgG isotype analysis.

结果 扩增的BCSP31保护性抗原基因片断在牛、羊和猪布氏杆菌株中具有高度保守性，构建的BCSP31/pVAX1重组表达质粒在COS7细胞有目的蛋白抗原的表达。

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI （http:///www.ncbi.nlm.nih.gov/）, ExPaSy （http://www.expasy.org/） and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心（NCBI，http：∥www.ncbi.nlm.nih.gov/）和瑞士生物信息学研究所的蛋白分析专家系统（ExPASY，http：∥ca.expasy.org/）中有关基因和蛋白的序列和结构信息分析的各种工具，结合其它生物信息学分析软件包，从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

Coll. Methods Insert the synthetic SARS-CoV N protein gene into vector pUC-18,ligate to plasmid pET28 and transform to E.

方法将已合成的SARS-CoV N蛋白基因片段，克隆至pUG18载体，与pET28质粒连接，转化大肠杆菌，进行SARS-CoV核衣壳融合蛋白表达。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失