质粒

Linear and circular plasmids were investigated among the 44 strains of the rice plant endophytic Streptomyces.

以广东番禺和五山地区水稻植株中分离到的内生链霉菌为对象，调查可能存在的内源质粒。

Methods Gene analysis by analysis susceptibility, ERIC-PCR techniquea and plasimid fingerprinting were put into practice for the detection of gene variation of 10 strains of Salmonella enteritidis. The similarities between different strains were obtained by software NTSYS-PS 2.0 for cluster analysis.

药敏试验采用K-B法，用碱裂解法提取细菌的质粒DNA，采用肠杆菌重复基因间隔重复共有序列技术对10株肠炎沙门菌进行基因分析，运用NTSYS-PC2.0软件进行同源性比较，聚类分析。

Existing plasmids and inherited materials are used to construct expression vector pZMW which could be integrated into S. erythraea chromosome and multi-copy expression vector pZM.

本实验室利用现有的质粒和遗传材料，构建了糖多孢红霉菌染色体整合型表达载体pZMW和游离型多拷贝表达载体pZM。

The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.

游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒，带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子，载体大小9880bp。

CTP gene was expressed in the erythrocytic stages of Plasmodium falciparum (FCC 1/HN) and eukaryotic expression vector of CTP was successfully constructed.

结论CTP基因在恶性疟原虫FCC1/HN株红细胞内期表达并成功地构建了CTP基因的重组表达质粒。

The hEGF was detectable in the supernatant of the modified keratinocytes. CONCLUSION The human keratinocytes modified with exogenetic hEGF cDNA can express and secrete hEGF.

由真核表达质粒pcDNA3-hEGF转染人角朊细胞获得的含外源性hEGF基因的角朊细胞具有表达分泌 hEGF的功能。

1,After transfected the mutated mtDNA of colorectal carcinoma,the mtDNA D-loop region of the transfected cells displays new mutation points.2,The external source pieces of the mutated mtDNA can integrate to nuclear genome after transfection.3,There's no differences in apoptosis between combinations after transfected the mutation of mtDNA in NIH3T3 and LST cells.4,The mutated mtDNA may affect the action mechanism of occurrence and development in colorectal carcinoma through affecting its mtDNA mutation or integrating exogenetic mtDNA to its nuclear which may cause the abnormal expression of oncogene or anti-oncogene.

（1）转染突变的大肠癌细胞mtDNA后转染细胞的mtDNA均可发生多处的突变位点。（2）通过转染后突变的外源性的mtDNA可以整合到核基因组内。（3）突变的mtDNA转染LST细胞及NIH3T3细胞后，不影响转染细胞的凋亡改变。（4）mtDNA的突变可能通过影响体细胞mtDNA的突变和通过外源性mtDNA在核内的整合从而影响癌基因或抑癌基因的表达异常，从而参与肿瘤的发生发展。线粒体DNA；D―环区；突变；质粒；pcDNA3.1；转染

Based on the study, it is very convenient for making a deep research of the foreign gene expression under the regulation of CSN2 flanking region.

为进一步利用 CSN2 质粒两端的调控序列，插入新的基因，研究其表达功能，或者进行乳腺生物反应器的研究奠定了基础。

The fusion expression vector and the non-fusion expression vector were constructed by the open reading frame of annfaf expressed specifically in strawberry Fragaria ananassa Duch.

将草莓？穴Fragaria ananassa Duch。？雪果实中特异表达的膜联蛋白基因annfaf的cDNA连接重组到pET-30a质粒中，构建了融合和非融合蛋白表达载体。annfaf 基因转化大肠杆菌E。

Suppression subtractive hybridization cDNA plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp.

构建了雌核发育银鲫原肠期胚胎和尾芽期胚胎间的抑制性差减杂交cDNA质粒文库。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！