质粒

The complex exhibits efficient cleaving activity in the presence of 〓.

在〓的存在下，它能使质粒DNA发生单链的完全裂解。

YIp5 plasmid which is replicable in E. coli but not in yeast is served as clonic carrier and ARS sequence in total DNA of rice cloned by air gun experiment.

以能在大肠杆菌复制，但不能在酵母菌中复制的YIp5质粒作为克隆载体，鸟枪法克隆了水稻总DNA中的ARS顺序。

Objective To construct prokaryotic recombinant plasmids of transcriptional co-activator gene of Clonorchis sinen sis, express and purify the recombinant protein and analyze its biological function.

摘要目的构建华支睾吸虫成虫转录辅激活因子(transcriptionalcoactivator ，TC)基因原核重组质粒，进行原核表达、鉴定及生物功能分析。

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis.

质粒DNA图谱分析为从分子水平上进行鸡大肠杆菌病流行病学调查提供了基础材料。

Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

With the comparition of the two groups, the conclusion can be drawn that the p80 protein expressed by the plasmid pEGFP-P80 cannot cause the detectable depression of activity of PK-15 cells.

结果表明，利用pEGFP-P80 重组表达质粒在PK-15 细胞中表达出的p80 蛋白未对宿主细胞有明显的致病变作用。

The result suggests that Al2O3 nanoparticle can stimulate donor function and thus result in a significant increase in conjugative plasmid dissemination in aquatic environments.

在一定条件下，纳米材料可促进质粒在液相中的接合转移，从而可能引发纳米材料的水环境安全问题。

The development of research strategies and novel molecule methods in recent years have made a more careful analysis of HGT possible under natural and artificial conditions. Furthermore, horizontal transfer of catabolic gene in environment enhances biodegradation of recalcitrant organic compounds. Particularly, the delivery of catabolic gene information of conjugative plasmids to bacterial population in situ has great potential in bioenhancement.

近年发展起来的研究策略和新的分子生物学方法使人们能对自然和人工模拟状态下的水平基因转移进行更精细的分析，降解基因在环境中的水平传播促进了难降解有机物的生物降解，尤其是携带降解基因的接合质粒向原位细菌种群中的转移在生物强化中具有巨大的潜力。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！