质粒

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

Coli.Methods The cDNA encoding CP was amplified by RT-PCR using the mRNA extracted from premature Carica papaya as a template,and inserted into vector pET22-b.

方法从生番木瓜中提取mRNA后进行反转录，得到木瓜凝乳蛋白酶的cDNA，PCR产物克隆到pET22-b载体中，重组质粒转化至大肠杆菌BL21，进行诱导表达及表达产物的检测。

Acremonium. However, low frequency of integrative genetic transformation is a major obstacle to gene cloning in Cephalosporium acremonium, for example, screening and identification of genes from a DNA bank by complementation of blocked mutants, knock out or replacement of genes.

过去的一些研究表明质粒对顶头孢霉的转化率太低，限制了许多分子生物学技术的应用，如利用互补突变株从顶头孢霉的DNA文库筛选和鉴别基因、基因敲除或者基因替换。

Objective: To construct and identify the chimeric MS/hIL12 eukaryotic expression plasmid.

目的：克隆含信号肽的Mtb8.4/hIL12嵌合基因，构建其真核表达质粒，并进行鉴定。

Aim To construct eukaryotic expression vector containing chimeric gene G1S0.7 of Hantavirus and express it in eukaryocyte.

目的 构建含汉坦病毒嵌合基因G1S0.7的真核重组质粒，并在真核细胞中有效表达。

Objective To construct the chimeric plasmid pcDNA3/HBc/B7.1 and detect the expression of the plasmid in vitro.

目的 构建HBV核心蛋白和B7.1分子真核表达嵌合质粒，并检测其在体外的表达。

Methodological design, synthesis is aimed at anoxic and revulsive factor - the nucleotide of few of particularity short cable length of 1 α is acerbity, compose builds anoxic and revulsive factor - of ShR-NA of 1 α particularity recombine plasmid, stability turns catch; of cell of colonic cancer SW 480 to use chloridize cobalt preparation is anoxic and revulsive education radical, imitate tumour is anoxic condition.

方法设计、合成针对缺氧诱导因子-1α的特异性短链寡核苷酸，构建缺氧诱导因子-1α特异性shR-NA的重组质粒，稳定转染结肠癌SW 480细胞；采用氯化钴制备缺氧诱导培养基，模拟肿瘤缺氧状态。

In order to isolate specific functional genes involved in reproduction and endocrine regulation and to reveal their evolutionary mechanisms in Chinese sturgeon, a chondrostean fish with a history of 140 million years, we constructed its pituitary cDNA library from a 4 year-old male.

为了能在中华鲟中发现与生长和生殖内分泌调控相关的基因，我们构建了中华鲟垂体的SMART cDNA质粒文库。

Methods Trizol reagent from clamworm digestive tract epithelial cells extracted methods Total RNA. The mRNA was purified and reverse transcripted into cDNA. The cDNA was linked into plasmid pDNR-Lib after digested by nucleate endonuclease Sfi I to construct cDNA library.

分离沙蚕消化道上皮细胞，使用Trizol试剂提取总RNA，纯化mRNA，用MMLV逆转录成cDNA，用Sfi I核酸内切酶酶切后连接到pDNR-Lib质粒中，构建cDNA文库。

Small interference RNA homolog to silenced gene in sequenceis indued into cell to clearage the mRNA of target gene. Many studies havedemonstrated that RNAi is an effective tool for gene diagnosis and gene therapy.

我们根据文献报道选择EZH2基因cDNA的461～481碱基为靶序列，构建EZH2-siRNA表达质粒，并研究其对人膀胱癌EJ细胞EZH2基因表达及生长抑制作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！