质粒

Elimination of conjugative drug resistance plasmids and nonconjugative drug resistanceplasmids from 20 and 19 Shigella strains respectively by norfloxacin and berberine in vitro were analysed.

用氟哌酸和黄连素对20株携有接合传递耐药性质粒及19株携有非接合传递耐药性质粒志贺氏菌进行体外消除研究。结果显示，可被消除的耐药性质粒大多数在24小时内被消除。

The range of MIC90 was-10 μg/ml. The susceptibility rates of the strains to other 10 agents were nearly or equal to 0%. Different plasmid profile strains had almost the same antimicrobial susceptibilities. 25 strains (including 18 strains harboring 34-80×106 plasmids) selectively examined to have no self-transmissible conjugative plasmid.

88.6%，MIC90为-10 μg/ml），对卡那霉素等其余10种药物的敏感率很低或不敏感，不同质粒谱菌株对药物的敏感性几乎相同，对包括 18株含34-80×106质粒在内的 25株菌进行接合转移试验，未检出传递性耐药性质粒。

Objective Establishment of a hybridoma cell line secreting a monoclonal antibody to facilitate iˉdentification of human tissue kallikrein（KLK1gene,hK1protein）.Methods Mice were immunized with E.coli-exˉpressed GST-hK1fusion protein and their spleen cells were fused with SP2/0myeloblastoma cells.Specificity of the monoclonal antibody was shown by Western blotting and by immunofluorescence.Results The monoclonal antibody reacted specifically to E.coli-expressed hK1and with the KLK1cDNA-transfected COS-1cells.

目的 将人组织激肽释放酶（基因命名 KLK1，酶命名为hK1）cDNA克隆入肠杆菌表达质粒，以重组菌表达的GST-hK1融合蛋白免疫小鼠，获得了分泌hK1特异单克隆抗体的杂交瘤细胞株方法将KLK1cDNA克隆入真核表达质粒，用获得的重组质粒转染COS-1细胞，经间接免疫荧光试验证明，上述单克隆抗体能与转染细胞发生特异反应。

The construction process includes the following steps: constructing recombinant plasmid containing phhA and phhB; transforming the recombinant plasmid to hygrophilous aeromonad; and screening recombinant hygrophilous aeromonad with the recombinant plasmid.

该重组菌中含有β-酮硫解酶基因phbA和乙酰乙酰辅酶A还原酶基因phbB。其构建方法包括以下步骤：1构建含phbA和phbB基因的重组质粒；2转化该重组质粒到嗜水气单胞菌中；3筛选出含有重组质粒的重组嗜水气单胞菌。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

Subtilis BD 224, it can be inferred that the host inheritable background could affect the segregational stability of plasmid.

这些起源相同的质粒在Baci-llus.subtilis ASl.1176中的分离稳定性存在差异，这种差异与质粒的大小和复制方式无关，而与质粒的拷贝数有一定的关系。

Expression of Bilirubin Oxidase cDNA from Myrothecium Verrucaria in E. coliThe 1. 6kb bilirubin oxidase cDNA from Myrothecium Verrucaria was excised from plasmid pGEM-T/BOX, and inserted into expression vector, pET-3a which were under the control of a T7 promoter.

用限制性内切酶将1.6kb疣疱漆斑菌胆红素氧化酶cDNA从质粒pGEM-T／BOX切下，插入到表达质粒pET-3a的T7启动子下游，构建成疣疱漆斑菌胆红素氧化酶表达质粒pET-3a／BOX。

In this study, the replicon of the largestdetected plasmid pBMB 165 and the smallest plasmid pBMB 175 of strain YBT-1765 werecharacterized.

该菌株至少含有三个内生质粒，本文对其可检测到的最大质粒pBMB165的复制区和最小质粒pBMB175的研究结果如下： 1。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Three plasmids named pBSGt pBSGd and pBSGk were constructed respectively with two plasmids named pEGH and pBS305. The plasmid pEGH contained gfp gene and pBS305 is an E. coli -Rhodococcus shuttle vector. These constructed plasmids can be used to detect the efficiency of promoter.

以带有报告基因gfp的质粒pEGH和大肠杆菌-红球菌穿梭载体pBS305为出发质粒，构建了三个带有不同启动子的表达质粒pBSGt、pBSGd和pBSGk，电击转化红平红球菌LY822。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！