质粒

Furthermore antisense plant expression vector of this gene was constructed using the pBI121 vector, laying foundation for studies on antisense regulation of ginseng β-amyrin synthase gene.

在此基础上，利用pBI121质粒载体，构建了人参β香树素合成酶基因的反义植物表达载体，为这一基因的反义调控研究打下基础。

The results suggested that RNA interference could inhibit AR in DPCs, and it might be significant for the androgenic alopecia gene therapy.

AR的RNA干扰质粒能抑制DPC内AR的表达，该实验为雄激素性脱发的基因治疗的进一步研究提供了必要的基础。

After anesthetization of rabbits in gene groups, recombinant plasmid DNA combined with transforming growth factor-β1 (TGF-β1) was injected to C2-3, C3-4 and C4-5 intervertebral disc (20μL per intervetebral disk).

基因治疗组家兔麻醉后，将带有转化生长因子β1基因的重组质粒DNA注入C2～3，C3-4，C4-5椎间盘中(每个椎间盘用量为20μL)。

Part Ⅲ: Animal immunization and anticarious experiment of the glucan binding protein B Plasmid pcDNA3. 1 -gbpB DNA vaccine was delivered into Sprague-Dawley rats in two different routes: intranasal and targeted salivary gland immunization . The dynamic variety of specific antibodies in sera and saliva were checked by ELISA.

第三部分 DNA防龋疫苗免疫动物及抑龋实验研究大量制各重组质粒pcDNA3.1-gbpB，分鼻腔滴注组、颌下腺区皮下注射组免疫SD大鼠，并设对照组和空白组，采用ELISA法检测血清及唾液中抗体水平的变化情况。

The antifreezing experiments on the transgenic plantlets proved that there was obvious difference of growth between the transgenic and nontransgenic plantlets at -5℃.

PCR检测表明，转化植株的条带和质粒中的目的条带一致，而未转化植株没有扩增出任何条带，说明外源基因已经整合到草莓基因组上。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

Transgenic expression of IL-1ra and degree of audiogenic seizure of P77PMC rats were observed after infection with pHSV-IL-1ra virus (〓 virus particles) by i. c. v injection and hippocampus injection. Results show: 1. Overexpression of IL-1ra by pHSV-IL-1ra significantly inhibited seizure attacks of P77PMC rat.

实验结果提示IL-1β可导致神经细胞NMDAR mRNA表达及受体活性增加，从而使其兴奋性增高；脑中内源性IL-1系统的异常很可能是形成P77PMC遗传癫痫易感大鼠惊厥易感性的重要因素之一；我们首次构建并鉴定了pHSV-IL-lra重组质粒，并成功地进行了脑内基因转移，使P77PMC大鼠的惊厥程度明显降低。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Borrelia burgdorferi Recombinant plasmid DNA sequence analysis Expression

姆病螺旋体；重组质粒； DNA序列分析；诱导表达

Methods We used the automatic DNA sequencing machine (Model 377) to detect the nucleotide sequence of the inserted part of the recombinant plasmid pBX1 from Borrelia burgdorferi B31 strain.

方法采用377型DNA自动测序仪对莱姆病螺旋体重组质粒pBX1的插入片段进行DNA序列测定，并通过计算机软件对其进行限制性内切酶酶谱分析。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！