质粒

Recombinant plasmid pEGFP-C1-GnRH/TRS showed good genetic stability in HeLa cells.

质粒pEGFP-G1-GnRH/TRS在HeLa细胞中有较好的遗传稳定性。

The CCND2 expression cell proliferation, cell cycle and cell apopotosis of the transfected LP-1 cells were studied by RT-PCR, trypanosome staining, flow cytometry and annexin V assay.

设计、合成CCND2短发夹RNA双链DNA模板，构建表达质粒pGenesil-2／CCND2，转染LP-1细胞，以RT—PCR检测对CCND2表达的抑制作用，以锥虫蓝染色计数和MTI检测细胞增殖，以Annexin V检测细胞凋亡，流式细胞术检测细胞周期。

In this experiment, His 5 gene was inserted into Ura 3 gene and constructed a new plasmid pLRH33, which contained a complete His 5 gene and partial Ura 3 gene at the ends of His 5 gene.

实验将His 5基因插入到一个Ura 3基因的中间，构建了一个新的质粒pLRH33，从而打断了Ura 3 基因使之不能表达。

Methods: The coding region of superoxide dismutase was amplified using PCR method from the E.co1i genome. The PCR product was cloned into PUC19-T vector and sequenced. In addition, the cloned coding region of Mn-SOD was inserted into the expression vector PET-28a to form the recombinant plasmid PET-28a-Mn-SOD and was then transformed into E.coli BL21 for expression.

用PCR方法从大肠杆菌2号基因组中扩增Mn-SOD基因编码区，克隆到pUCm-T Vector，测定核苷酸序列；再将基因编码区克隆到原核表达载体PET-28a，构建含Mn-SOD基因的重组表达质粒，转化到大肠杆菌BL21中进行IPTG诱导表达。

Fourth, we constructed pBES-CFA/I vectorial vaccine and test the expression of CFA/I by SDS-PAGE.

第四、构建了pBEGFP-CFA/I 载体疫苗表达质粒，并进行了SDS-PAGE检测。

Reverse Transcription-Polymerase Chain Reaction was used to clone the whole-length gene of BMP-2 and construct AdV vector.

应用反转录-聚合酶链反应法克隆出骨形态发生蛋白质类2全长基因，构建重组腺病毒载体，DNA-磷酸钙共沉法将辅助质粒PJM17转染293细胞，同源重组构建出重组腺病毒。

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达，从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221，转化大肠杆菌DH5α，筛选并鉴定阳性克隆。

Oral administration of DNA nanoparticles synthesized by complexing pCMVβ DNA with chitosan,which modified with gelatin,Sodium Alginate,PEG and acetylize respectively,resulted in transduced gene expression in the intestinal epithelium.

分别使用明胶、海藻酸钠、PEG及乙酰化修饰包裹含LacZ的质粒pCMVβ的壳聚糖纳米颗粒，通过口服发送后经X-gal染色检测目的基因在小鼠体内的表达。

It was identified that the nif gene for aerogenic P. agglomerans strain waslocated on plasmid while for anaerogenic strains were located on chromosomes usingKlebsiella pneumoniae nifHDK as probe.

用nifHDK为探针进行Southern杂交证明固氮成团泛菌产气型的菌株固氮基因定位在质粒上，而不产气型菌株固氮基因定位在染色体上。

A new method was adopted to study the amidation of HNP expressed as fused protein.

以此为基础构建了环化表达质粒，并对表达条件进行了探索。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！