质粒

Four stable cell lines and wild type CNE-2 cell line are inoculated to subcutis of nude mice, animal models are established.

将第二部分获得的4种稳定表达具有调控作用的miRNA质粒的CNE-2细胞株和野生型CNE-2细胞株，分别注入裸鼠皮下，成功建立了人鼻咽癌裸鼠移植瘤模型。

Sulfuration is commonly found on the surface of the minerals.

质粒DNA评价结果表明，沙尘暴颗粒物对DNA的损伤明显低于非沙尘暴期颗粒物，PM2.5对DNA的损伤明显高于PM10，北京市大气颗粒物的生物活性主要来源于其中的水溶组分，尤其是水溶性Zn。

On the functional differentiation,we found and that the type II ras-GTPaseactivating protein (p100-GAP),which was specifically expressed in humanplacenta,was largely expressed in the highly-differentiated syncytiotrophoblastcells and moderately expressed in the cultured intermediate trophoblast cells.While in the non-differentiated cytotrophoblast cell line-NPC,no expression was observed.Its mRNA and protein expressingcharacterization was in consistent with that of hCGβ,which was one of the mostimportant markers of the trophoblast cell functional differentiation.In addition,the expressing amount of p100-GAP increased with the progressing of pregnancyand the syncytium formation in vitro.

细胞功能分化的研究结果表明：经重组质粒的构建获得特异表达于人胎盘的p100－GAP的cDNA探针后，从蛋白和分子水平证实p100－GAP大量表达于分化程度高的合体滋养层细胞，少量表达于体外培养的中间型滋养层细胞，而非分化的细胞滋养层细胞NPC则不表达，这种表达特性与作为滋养层细胞功能分化重要指标之一的hCGβ高度一致；p100-GAP表达量还随妊娠过程和体外合体化过程而逐渐增加。

ABSTRACT Bacillus subtilis tryptophanyl-tRNA synthetase was expressed and purified by the aid of the high-level expr...

利用枯草杆菌色氨酰tRNA合成酶的高表达质粒pKSW1表达和纯化了枯草杆菌色氨酰tRNA合成酶。

Objective To search and identify novel gene by screening the cDNA library of adult Taenia saginata asiatica,and to clone and express the gene so as to lay a foundation for the further study of Ta CRISP gene's function.

目的 通过筛选亚洲牛带绦虫成虫cDNA 质粒文库，识别半胱氨酸分泌蛋白，并进行克隆表达，为进一步研究其功能提供基础依据。

Those expression plasmid were respectively transformed into E.coli DH5a, thenthe expression strains were induced for 5h under 42℃, SDS-PAGE confirmed that interestprotein was expressed by pBV220-R- IFN-γ_m strain and was 16kd in size. Its contentaccounted for 38.6％of the thalline protein.

分别转化大肠杆菌DH5α，42℃诱导表达4h，经SDS-PAGE电泳鉴定，原核表达质粒pBV220-R-IFN-γ_m表达的目的蛋白大小为16Kd，占细菌总蛋白的38.6％。

Aim To elusidate the effect of tissue-type plasminogen activator gene plasmid on prevention of the thrombosis after atherosclerosis.

目的 探讨组织型纤溶酶原激活物基因质粒在预防动脉粥样硬化及血栓形成中的作用。

To elucidate the transfection of tissue-type plasminogen activator gene to prevent vascular anastomotic restenosis after coronary bypass.

目的 探讨应用组织型纤溶酶原激活物基因质粒预防冠状动脉搭桥术后吻合口再狭窄。

Methods The expression plasmid pSVvWF containing full-length cDNA of vWF was used to site direct mutagenesis by polymerase chain reaction and transitorily expressed in the COS-7 cells, vWF:Ag in the supernatant and the cell lysate between wild type and pSVAla737Glu vWF were measured.

用聚合酶链反应的方法对野生型的vWF全长表达质粒进行体外定点诱变，在COS-7细胞中进行表达。

Objective To study the genetic stability of recombinant plasmid pEGFP-C1-GnRH/TRS in HeLa cells.

目的 研究质粒pEGFP-C1-GnRH/TRS在HeLa细胞中的遗传稳定性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！