质粒

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

Objective To analyze the plasmid profiles, drug resistant activities and genetically homologous correlationship of 130 strains of Shigella.

目的 分析志贺菌质粒谱、耐药性及流行菌株在遗传学上的同源关系。

For this aim, an I kappa B alpha-dominant negative plasmid was injected into the soleus muscle of rats.

对于这个目标，的iκ b的阿尔法-显性阴性质粒（俗称为superrepressor ）被注入到比目鱼肌中的作用。

As a carrier of plasmid DNA, dextran-spermine cationic polysaccharide was prepared by means of reductive-amination between oxidized dextran and the natural oligoamine, spermine.

作为质粒DNA的载体，阳离子的精胺多聚糖通过氧化右旋糖苷，精胺等之间的脱氨基化作用作用来制备。

As a carrier of plasmid DNA, dextran-spermine cationic polysaccharide was prepared by means of reductie-amination between oxidized dextran and the natural oligoamine, spermine.

作为质粒DNA的载体，阳离子的精胺多聚糖通过氧化右旋糖苷，精胺等之间的脱氨基化作用作用来制备。

RESULTS: Some cell colonies were detected at 4 days after pDC316-SOX-9 plasmid transfection. Spindle-shaped cells were collected after clone amplification.

结果：pDC316-SOX-9质粒转染4 d出现小片的细胞集落，挑取克隆扩增培养后，细胞呈梭形纤维状。

A 3-1E gene fragment was amplified from Eimeria acervulina SH strain sporozoite by RT-PCR,and it was cloned into eukaryotic expression vector pcDNA3.1 to construct the recombinant plasmid pcDNA3.1-3-1E.

应用反转录-聚合酶链式反应技术从堆形艾美球虫SH株子孢子中扩增3-1E基因片段，将其克隆入真核表达载体pcDNA3.1中，构建重组表达质粒pcDNA3.1-3-1E。

Methods Entire coding sequence of CSP was reverse transcribed and amplified by RT-PCR with sporozoite total RNA as template,then cloned into pFLAG-CMV8 for construction of the recombinant plasmid pFLAG-CMV8-CSP.

以约氏疟原虫BY265株子孢子总RNA为模板，用RT-PCR扩增CSP基因的编码区序列并克隆至pFLAG-CMV8载体，构建重组质粒pFLAG-CMV8-CSP。

Rabbit sternocleidomastoid muscle in vivo could absorb and express foreign plasmid,which meant a potential site of molecular surgery.

胸锁乳突肌可摄取和表达外源质粒，是一个潜在的分子外科转基因部位。

Objective To establish a simple,accurate and practi cal method of extracting plasmid from gram-negative and gram-positive bacteria ,Method we selected,Biswajit sahas method and comported others method, such as controlling pH andtemperature strictly and increasing EDTA-Na2 capacity.

为建立一种革兰氏阴性及阳性菌均能分析的质粒提取方法，以Biswajit saha的方法为基础，对比酸酚法、热变性法、碱变性法、酶法等方法，研制符合国内一般实验室的方法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！