质粒

The effect of frequency and length of square pulse,as well as eletroporation time on hFⅨ expression were investigated.

表达质粒G1NaMCⅨ肌肉转移和表达，并观察了方波频率、宽度、电击持续时间对hF。

Human bladder cancer cell line T24 was used, two different NF-kB inhibitors, pyrrolidine dithiocarbamate and dominant negative IkBα were used.

在人膀胱癌细胞系T24中，使用两个核转录因子kB抑制剂即显性失活IkBα质粒和二硫氨甲酸脂吡咯烷。

Cloning vector pEGFP-RECK was successfully constructed, which laid a foundation for further studying the function of RECK gene.

成功构建了GFP-RECK融合蛋白表达质粒，这为进一步研究该基因的功能奠定了基础。

Genes of HBV envprotein S was placed in this vector and expressed at sable and high level.

用它构建表达HBV包膜S蛋白的重组质粒，获得稳定的高表达。

Methods full length hbv-pol gene was amplified and was introduced into a high effective expression plasmid of saccharomyces cerevisiae.

将全长hbv-pol基因用pcr方法扩增并连接到在酿酒酵母高效表达的质粒中。

PCRs were performed on genomic DNAs of four pear cultivars Sand pear, Huangguan pear, Xueli pear, Yali pear using primers derived from sequences of previous published PPO. The target bands were recovered and purified. The fragments were ligated with the plasmid of pGEM-T Vector by the method of direct T-A cloning.

以4种不同来源的梨基因组DNA为模板，根据已经发表的多酚氧化酶基因序列设计引物，利用PCR技术，克隆到了鸭梨、雪花梨、砂梨、黄冠梨约1.8 kb的完整多酚氧化酶基因，将纯化后的扩增产物克隆到质粒pGEM-T vec-tor中，转化DH-5α菌，挑选阳性克隆进行测序。

Methods Six recombinant plasmids with shRNAs targeting the PDE5A3 gene of Homo sapien were constructed.

方法构建6个靶向人PDEsA3基因的短发夹RNA重组质粒，转染人阴茎海绵体平滑肌细胞48 h后，逆转录-聚合酶链反应及Western blot检测PDE5A3基因的表达抑制效果。

The α-AE_(75) cDNA was fused downstream of melC1 signal peptide encoding sequence in correct reading frame so that α-AE_(75) will be expressed secretively.

用这两个表达质粒分别转化S.lividans得到能分泌表达α-酰胺化酶的工程菌S.lividans [pIJmel／AE680]和S.lividans[pMS-mel／AE680]。

The 5＇coding region of its eDNA was optimized according to the codon bias of Pichia pastoris to make it secretively express in Pichia pastoris with higher performance. Two-step DNA synthsis was used to synthesize the eDNA sequence being optimized of ADTZ. OPT-ADTZ was connected with constitutive plasmid pGAPZaA to construct the reecombinant plasmid pNOA. pNOA was linearized and then transformed into Pichia pastoris GS115. Then codonoptimized ADTZ was constitutively and secretively expressed in Pichia pastoris.

为了使重组蛋白rADTZ能在毕氏酵母中高效分泌表达，根据毕氏酵母密码子的偏好性对rADTZ的5'末端的编码区域进行了优化，利用two-stepDNAsynthsis技术合成出ADTZ优化的基因序列OPT-ADTZ，并与组成型表达载体pGAPZaA连接，构建重组质粒pNOA，线性化pNOA后转化至毕氏酵母GS115中，实现了密码子优化的rADTZ组成型分泌表达。

Because many of these sequences are not in GenBank, a BLAST serer has been added; another new feature is an abbreiated alignment for the tRNA-like domain only.

由于其中的许多序列没有被GenBank收录，所以添加了一个Blast服务；新添加了许多来源于质粒的tmRNA序列，其中的5个是从公共数据库中收集而来，还有十条是直接测序得到的。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！