质粒

A plastid that contains pigments other than chlorophyll, usually yellow or orange carotenoids.

有色体，色素体除了叶绿素外的含色素的质粒，通常为黄色或橙色的类胡萝卜素

In the biotechnology area, we has set up polytype Handson yeast express system which owned freedom intellectual property rights, including:(1) URA3 - defective nutrition host cell strain HU-II;(2) secretion and intracellylar expressing plasmid carrier pMPT-01、pMPT-02;(3) a platform for semi-works

在生物技术领域：建立了拥有自主知识产权、较为完善的多型汉逊酵母表达系统，包括：（1）URA3 -营养缺陷型宿主细胞株HU-II；（2）分泌型和胞内型表达质粒载体pMPT-01、pMPT-02；（3）汉逊酵母细胞电穿孔转化操作程序及多拷贝、稳定化整合转化子筛选程序；（4）中试生产规模的发酵和纯化平台。

Objective To obtain the functional fusion protein of rhoptry protein 2(ROP2) and major surface antigen 1 of Toxoplasma gondii in order to further prepare for the diagnosis and polyvalent vaccines of toxoplasmosis.

目的构建编码弓形虫RH株棒状体蛋白2(ROP2)和主要表面抗原1的重组表达质粒，纯化和复性的融合蛋白为弓形虫病快速诊断试剂盒及蛋白质疫苗的研制作准备。

The expressed proteins were purified on 15% SDS-PAGE, the specific protein gel was cut down, transferred onto nitrocellulose membrane and stained with ponceau for 10 minutes.

结果 获得1株含HIV-1 gp41基因的重组质粒，其蛋白的特异性表达为8%，经HIV-1阳性血清检测和国家标准参比血清检定，该表达蛋白灵敏性、特异性均为100%。

In the study, Pristane was been used with recombinant BLyS and BLC vector those can expressed in vivo in mice, the influence to SLE-like disease was investigate. Pristane associated with BLyS or BLC aggravates proteinuria. This fact indicates that the abnormal expression of the two genes has definite effect on mice lupus-like disease process.

本研究中，当Pristane与可在体内表达的重组BLyS或BLC基因质粒合用时，小鼠的蛋白尿明显加重，表明这两个基因的异常表达对小鼠的狼疮样病变过程有明显的影响。

Objective: To construct prokaryotic expression plasmid of VEGF-SEA gene, and to get the fusion proteins.

目的：构建血管内皮生长因子－葡萄球菌肠毒素A基因的原核表达质粒，获得VEGF-SEA融合蛋白。

The prokaryotic expression vector of Aβ42 subunit vaccine was successfully constructed.

已成功地构建了Aβ42亚单位疫苗的原核表达质粒。

Recombinant expression vector k14/c-myc-paav was constructed bearing the mouse c-Myc cDNA derived by the skin specific human keratin 14 promoter, then the linear vector was microinjected into the two pronucleus of the C57BL/6 fertilized eggs to produce transgenic mice.256 microinjected eggs were transferred, 6 of the foster mothers were pregnant and 44 adult mice were produced, two of them were positive transgenic mice confirmed by PCR and Southern blot analysis, transgenic rate was about 4%.

以CMV-PAAV载体为基础载体，以皮肤特异人K14（人角蛋白14）基因启动子替换原质粒中的CMV启动子后，在其多克隆位点区依次插入鼠c-Myc基因的cDNA序列和gfp基因的编码序列，构建了高效表达c-Myc基因的真核表达载体。通过双原核显微注射技术，将线性化的载体DNA注入到C57BL/6小鼠受精卵的两个原核中，然后将注射后状态良好的胚胎移植到代孕鼠的输卵管中，共移植了256枚受精卵，6只受体怀孕，最终获得44只成年小鼠。

Thereafter,DNA fragment of Hyl-1-Amp-Hyl-1 was subcloned into pBR322 vector,the resulting construct was then checked by PCR and restriction analysis for the proper configuration of the knockout vector pBR322-SA.

该载体与质粒pBR322分别用EcoRⅠ、PstⅠ双酶切后进行连接，得到基因敲除的重组载体pBR322SA，PCR 及限制性酶切分析，构建的敲除载体与设计结果相符。

Protamine is an inhibitor of angiogenesis, Nature,

细胞可传代培养而不发生质粒的丢失；但是基因转移效率较低。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！